Auxotrophic-precursor directed biosynthesis of nonribosomal lipopeptides with modified tryptophan residues

Bagher Amir-Heidari; Jenny Thirlway; Jason Micklefield

doi:10.1039/b718766c

Auxotrophic-precursor directed biosynthesis of nonribosomal lipopeptides with modified tryptophan residues

Bagher Amir-Heidari, Jenny Thirlway, Jason Micklefield

Research output: Contribution to journal › Article › peer-review

Abstract

Feeding 5-hydroxy and 5-fluorotryptophan to a Streptomyces coelicolor Trp-auxotrophic strain WH101 results in the production of a number of new calcium-dependent antibiotics (CDAs) possessing modified Trp residues. It is anticipated that this method could be used to modulate the biological properties of Trp-containing nonribosomal peptide natural products, or to generate analogues with useful fluorescent properties for studying biological mechanisms of action. © 2008 The Royal Society of Chemistry.

Original language	English
Pages (from-to)	975-978
Number of pages	3
Journal	Organic and Biomolecular Chemistry
Volume	6
Issue number	6
DOIs	https://doi.org/10.1039/b718766c
Publication status	Published - 2008

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title = "Auxotrophic-precursor directed biosynthesis of nonribosomal lipopeptides with modified tryptophan residues",

abstract = "Feeding 5-hydroxy and 5-fluorotryptophan to a Streptomyces coelicolor Trp-auxotrophic strain WH101 results in the production of a number of new calcium-dependent antibiotics (CDAs) possessing modified Trp residues. It is anticipated that this method could be used to modulate the biological properties of Trp-containing nonribosomal peptide natural products, or to generate analogues with useful fluorescent properties for studying biological mechanisms of action. {\textcopyright} 2008 The Royal Society of Chemistry.",

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AU - Amir-Heidari, Bagher

AU - Thirlway, Jenny

AU - Micklefield, Jason

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N2 - Feeding 5-hydroxy and 5-fluorotryptophan to a Streptomyces coelicolor Trp-auxotrophic strain WH101 results in the production of a number of new calcium-dependent antibiotics (CDAs) possessing modified Trp residues. It is anticipated that this method could be used to modulate the biological properties of Trp-containing nonribosomal peptide natural products, or to generate analogues with useful fluorescent properties for studying biological mechanisms of action. © 2008 The Royal Society of Chemistry.

AB - Feeding 5-hydroxy and 5-fluorotryptophan to a Streptomyces coelicolor Trp-auxotrophic strain WH101 results in the production of a number of new calcium-dependent antibiotics (CDAs) possessing modified Trp residues. It is anticipated that this method could be used to modulate the biological properties of Trp-containing nonribosomal peptide natural products, or to generate analogues with useful fluorescent properties for studying biological mechanisms of action. © 2008 The Royal Society of Chemistry.

U2 - 10.1039/b718766c

DO - 10.1039/b718766c

M3 - Article

SN - 1477-0520

VL - 6

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EP - 978

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 6

ER -

Auxotrophic-precursor directed biosynthesis of nonribosomal lipopeptides with modified tryptophan residues

Abstract

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