Bacterial detection using carbohydrate-functionalised CdS quantum dots: a model study exploiting E. coli recognition of mannosides

B. Mukhopadhyay, M.B. Martins, R. Karamanska, D.A. Russell, R.A. Field

Research output: Contribution to journalArticlepeer-review

Abstract

Mannose-coated CdS quantum dots (Man-QDs) were prepared in a facile aqueous, one-pot process that exploits the self-assembly of thiolated mannose in the presence of CdS under reducing conditions. The resulting ∼15 nm diameter nanoparticles produce an intense, broad luminescence emission centred at 550 nm. These Man-QDs induce luminescent aggregates of Escherichia coli which can be used to detect bacteria in cell suspensions containing as few as 104 E. coli per mL. The aggregation process is dependent on the E. coli cell surface FimH mannose-specific lectin. The recognition and subsequent detection of the E. coli using the Man-QD has been shown to be specific as aggregation does not occur either with an E. coli strain defective in the FimH lectin or with galactose-coated QDs. © 2008 Elsevier Ltd. All rights reserved.
Original languageUndefined
Pages (from-to)886-889
Number of pages4
JournalTetrahedron Letters
DOIs
Publication statusPublished - 2009

Research Beacons, Institutes and Platforms

  • Manchester Institute of Biotechnology

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