Baseline proteomics characterisation of the emerging host biomanufacturing organism Halomonas bluephagenesis

Matthew Russell, Andrew Currin, William Rowe, Guo-qiang Chen, Perdita Barran, Nigel S. Scrutton

Research output: Contribution to journalArticlepeer-review


Despite its greener credentials, biomanufacturing remains financially uncompetitive compared with the higher carbon emitting, hydrocarbon-based chemical industry. Replacing traditional chassis such as E. coli with novel robust organisms, are a route to cost reduction for biomanufacturing. Extremophile bacteria such as the halophilic Halomonas bluephagenesis TD01 exemplify this potential by thriving in environments inherently inimical to other organisms, so reducing sterilisation costs. Novel chassis are inevitably less well annotated than established organisms. Rapid characterisation along with community data sharing will facilitate adoption of such organisms for biomanufacturing. The data record comprises a newly sequenced genome for the organism and evidence via LC-MS based proteomics for expression of 1160 proteins (30% of the proteome) including baseline quantification of 1063 proteins (27% of the proteome), and a spectral library enabling re-use for targeted LC-MS proteomics assays. Protein data are annotated with KEGG Orthology, enabling rapid matching of quantitative data to pathways of interest to biomanufacturing.
Original languageEnglish
Article number492
JournalScientific Data
Issue number1
Early online date13 Aug 2022
Publication statusPublished - 13 Aug 2022

Research Beacons, Institutes and Platforms

  • Manchester Institute of Biotechnology


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