Beta-adrenoceptor blockade markedly attenuates transgene expression from cytomegalovirus promoters within the cardiovascular system

Husein K. Salem; Parisa Ranjzad; Anita Driessen; Clare E. Appleby; Anthony M. Heagerty; Paul Kingston

doi:10.1161/01.ATV.0000239445.67579.19

Beta-adrenoceptor blockade markedly attenuates transgene expression from cytomegalovirus promoters within the cardiovascular system

Husein K. Salem, Parisa Ranjzad, Anita Driessen, Clare E. Appleby, Anthony M. Heagerty, Paul Kingston

Research output: Contribution to journal › Article › peer-review

Abstract

OBJECTIVES - The major immediate-early cytomegalovirus enhancer/promoter (MIECMV), widely used in cardiovascular gene therapy, contains several positively regulatory cAMP response elements (CRE). Catecholamine signaling via β-adrenoceptors might increase transgene expression from MIECMV, and if so, β-blockers may have a detrimental effect on the efficacy of clinical cardiovascular gene therapy strategies. METHODS AND RESULTS - Cultured smooth muscle cells were exposed to isoprenaline, atenolol, or propranolol, alone and in combination before infection with adenoviruses expressing β-galactosidase. β-galactosidase expression was assayed 72 hours later. Isoprenaline increased transgene expression from MIECMV up to 8-fold (P

Original language	English
Pages (from-to)	2267-2274
Number of pages	7
Journal	Arteriosclerosis, Thrombosis, and Vascular Biology
Volume	26
Issue number	10
DOIs	https://doi.org/10.1161/01.ATV.0000239445.67579.19
Publication status	Published - Oct 2006

Keywords

β-adrenergic receptor blockers
Catecholamines
Gene therapy

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10.1161/01.ATV.0000239445.67579.19

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title = "Beta-adrenoceptor blockade markedly attenuates transgene expression from cytomegalovirus promoters within the cardiovascular system",

abstract = "OBJECTIVES - The major immediate-early cytomegalovirus enhancer/promoter (MIECMV), widely used in cardiovascular gene therapy, contains several positively regulatory cAMP response elements (CRE). Catecholamine signaling via β-adrenoceptors might increase transgene expression from MIECMV, and if so, β-blockers may have a detrimental effect on the efficacy of clinical cardiovascular gene therapy strategies. METHODS AND RESULTS - Cultured smooth muscle cells were exposed to isoprenaline, atenolol, or propranolol, alone and in combination before infection with adenoviruses expressing β-galactosidase. β-galactosidase expression was assayed 72 hours later. Isoprenaline increased transgene expression from MIECMV up to 8-fold (P",

keywords = "β-adrenergic receptor blockers, Catecholamines, Gene therapy",

author = "Salem, {Husein K.} and Parisa Ranjzad and Anita Driessen and Appleby, {Clare E.} and Heagerty, {Anthony M.} and Paul Kingston",

year = "2006",

month = oct,

doi = "10.1161/01.ATV.0000239445.67579.19",

language = "English",

volume = "26",

pages = "2267--2274",

journal = "Arteriosclerosis, Thrombosis and Vascular Biology",

issn = "1079-5642",

publisher = "Lippincott Williams & Wilkins",

number = "10",

}

TY - JOUR

T1 - Beta-adrenoceptor blockade markedly attenuates transgene expression from cytomegalovirus promoters within the cardiovascular system

AU - Salem, Husein K.

AU - Ranjzad, Parisa

AU - Driessen, Anita

AU - Appleby, Clare E.

AU - Heagerty, Anthony M.

AU - Kingston, Paul

PY - 2006/10

Y1 - 2006/10

N2 - OBJECTIVES - The major immediate-early cytomegalovirus enhancer/promoter (MIECMV), widely used in cardiovascular gene therapy, contains several positively regulatory cAMP response elements (CRE). Catecholamine signaling via β-adrenoceptors might increase transgene expression from MIECMV, and if so, β-blockers may have a detrimental effect on the efficacy of clinical cardiovascular gene therapy strategies. METHODS AND RESULTS - Cultured smooth muscle cells were exposed to isoprenaline, atenolol, or propranolol, alone and in combination before infection with adenoviruses expressing β-galactosidase. β-galactosidase expression was assayed 72 hours later. Isoprenaline increased transgene expression from MIECMV up to 8-fold (P

AB - OBJECTIVES - The major immediate-early cytomegalovirus enhancer/promoter (MIECMV), widely used in cardiovascular gene therapy, contains several positively regulatory cAMP response elements (CRE). Catecholamine signaling via β-adrenoceptors might increase transgene expression from MIECMV, and if so, β-blockers may have a detrimental effect on the efficacy of clinical cardiovascular gene therapy strategies. METHODS AND RESULTS - Cultured smooth muscle cells were exposed to isoprenaline, atenolol, or propranolol, alone and in combination before infection with adenoviruses expressing β-galactosidase. β-galactosidase expression was assayed 72 hours later. Isoprenaline increased transgene expression from MIECMV up to 8-fold (P

KW - β-adrenergic receptor blockers

KW - Catecholamines

KW - Gene therapy

U2 - 10.1161/01.ATV.0000239445.67579.19

DO - 10.1161/01.ATV.0000239445.67579.19

M3 - Article

C2 - 16888240

VL - 26

SP - 2267

EP - 2274

JO - Arteriosclerosis, Thrombosis and Vascular Biology

JF - Arteriosclerosis, Thrombosis and Vascular Biology

SN - 1079-5642

IS - 10

ER -

Beta-adrenoceptor blockade markedly attenuates transgene expression from cytomegalovirus promoters within the cardiovascular system

Abstract

Keywords

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