Binding of a desmetallo-porphyrin conjugate of Hoechst 33258 to DNA. III. Strong binding to single-strand oligonucleotides

E. V. Bichenkova; S. Frau; O. S. Fedorova; K. T. Douglas

doi:10.1081/NCN-100001444

Binding of a desmetallo-porphyrin conjugate of Hoechst 33258 to DNA. III. Strong binding to single-strand oligonucleotides

E. V. Bichenkova, S. Frau, O. S. Fedorova, K. T. Douglas

Research output: Contribution to journal › Article › peer-review

Abstract

The binding of the conjugate of Hoechst 33258 with 5,10,15,20-tetrakis (1-methyl-4-pyridyl)-21H.23H-porphyrin (PORHOE) to single-strand DNA has been detected by UV-vis spectrophotometry and 1H-NMR. The red-shift of porphyrin Soret band with strong hypochromicity indicates that the porphyrin moiety dominates in the interaction of the PORHOE with ssDNA. The affinity constants of PORHOE for d(GCATACAATTCG) or d(CGAATTGTATGC) were determined to be >105 M-1, with strong cooperativity.

Original language	English
Pages (from-to)	157-168
Number of pages	11
Journal	Nucleosides, Nucleotides and Nucleic Acids
Volume	20
Issue number	1-2
DOIs	https://doi.org/10.1081/NCN-100001444
Publication status	Published - 2001

Keywords

Binding constant
Ligand
NMR
Single strand DNA

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title = "Binding of a desmetallo-porphyrin conjugate of Hoechst 33258 to DNA. III. Strong binding to single-strand oligonucleotides",

abstract = "The binding of the conjugate of Hoechst 33258 with 5,10,15,20-tetrakis (1-methyl-4-pyridyl)-21H.23H-porphyrin (PORHOE) to single-strand DNA has been detected by UV-vis spectrophotometry and 1H-NMR. The red-shift of porphyrin Soret band with strong hypochromicity indicates that the porphyrin moiety dominates in the interaction of the PORHOE with ssDNA. The affinity constants of PORHOE for d(GCATACAATTCG) or d(CGAATTGTATGC) were determined to be >105 M-1, with strong cooperativity.",

keywords = "Binding constant, Ligand, NMR, Single strand DNA",

author = "Bichenkova, {E. V.} and S. Frau and Fedorova, {O. S.} and Douglas, {K. T.}",

year = "2001",

doi = "10.1081/NCN-100001444",

language = "English",

volume = "20",

pages = "157--168",

journal = "Nucleosides, Nucleotides and Nucleic Acids",

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publisher = "Taylor & Francis",

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TY - JOUR

T1 - Binding of a desmetallo-porphyrin conjugate of Hoechst 33258 to DNA. III. Strong binding to single-strand oligonucleotides

AU - Bichenkova, E. V.

AU - Frau, S.

AU - Fedorova, O. S.

AU - Douglas, K. T.

PY - 2001

Y1 - 2001

N2 - The binding of the conjugate of Hoechst 33258 with 5,10,15,20-tetrakis (1-methyl-4-pyridyl)-21H.23H-porphyrin (PORHOE) to single-strand DNA has been detected by UV-vis spectrophotometry and 1H-NMR. The red-shift of porphyrin Soret band with strong hypochromicity indicates that the porphyrin moiety dominates in the interaction of the PORHOE with ssDNA. The affinity constants of PORHOE for d(GCATACAATTCG) or d(CGAATTGTATGC) were determined to be >105 M-1, with strong cooperativity.

AB - The binding of the conjugate of Hoechst 33258 with 5,10,15,20-tetrakis (1-methyl-4-pyridyl)-21H.23H-porphyrin (PORHOE) to single-strand DNA has been detected by UV-vis spectrophotometry and 1H-NMR. The red-shift of porphyrin Soret band with strong hypochromicity indicates that the porphyrin moiety dominates in the interaction of the PORHOE with ssDNA. The affinity constants of PORHOE for d(GCATACAATTCG) or d(CGAATTGTATGC) were determined to be >105 M-1, with strong cooperativity.

KW - Binding constant

KW - Ligand

KW - NMR

KW - Single strand DNA

U2 - 10.1081/NCN-100001444

DO - 10.1081/NCN-100001444

M3 - Article

SN - 1532-2335

VL - 20

SP - 157

EP - 168

JO - Nucleosides, Nucleotides and Nucleic Acids

JF - Nucleosides, Nucleotides and Nucleic Acids

IS - 1-2

ER -

Binding of a desmetallo-porphyrin conjugate of Hoechst 33258 to DNA. III. Strong binding to single-strand oligonucleotides

Abstract

Keywords

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