Binding of a porphyrin conjugate of Hoechst 33258 to DNA. II. NMR spectroscopic studies detect multiple binding modes to a 12-MER nonself-complementary duplex DNA

S. Frau, E. V. Bichenkova, G. A. Morris, K. T. Douglas

    Research output: Contribution to journalArticlepeer-review

    Abstract

    We have probed by 1H NMR spectroscopy the molecular basis of the interaction between Hoechst 33258 conjugated to a des-metalloporphyrin and a non self-complementary duplex DNA sequence, designed on the known chemical nuclease selectivity of this system. The imino NMR spectra are consistent with two distinct families of structure, that is, PORHOE binding either way along the duplex. 2D spectral, T2, and linewidth data suggest multiple species within the two conformational families.
    Original languageEnglish
    Pages (from-to)145-156
    Number of pages11
    JournalNucleosides, Nucleotides and Nucleic Acids
    Volume20
    Issue number1-2
    DOIs
    Publication statusPublished - 2001

    Keywords

    • DNA duplex
    • DNA-binding
    • Ligand
    • Minor groove
    • NMR
    • NOESY

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