Binding of an aptamer to the N-terminal fragment of VCAM-1

Fabien Chauveau; Youssef Aissouni; Jorg Hamm; Herve Boutin; Domenico Libri; Frederic Duconge; Bertrand Tavitian

doi:10.1016/j.bmcl.2007.09.046

Binding of an aptamer to the N-terminal fragment of VCAM-1

Fabien Chauveau, Youssef Aissouni, Jorg Hamm, Herve Boutin, Domenico Libri, Frederic Duconge, Bertrand Tavitian

Research output: Contribution to journal › Article › peer-review

Abstract

In vitro selection of 2'-fluoropyrimidine oligonucleotide aptamers was performed against the N-terminal two-domain fragment of mouse VCAM-1. The SELEX procedure enriched the starting pool in a family of homologous sequences. High binding affinity (10nM) of one member of this family, aptamer 12.11, was demonstrated in a filter binding assay.

Original language	English
Pages (from-to)	6119-6122
Number of pages	3
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	17
Issue number	22
DOIs	https://doi.org/10.1016/j.bmcl.2007.09.046
Publication status	Published - 15 Nov 2007

Keywords

Aptamer
SELEX
VCAM-1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.bmcl.2007.09.046

Cite this

@article{6589654766644bdebd4482d1346a9781,

title = "Binding of an aptamer to the N-terminal fragment of VCAM-1",

abstract = "In vitro selection of 2'-fluoropyrimidine oligonucleotide aptamers was performed against the N-terminal two-domain fragment of mouse VCAM-1. The SELEX procedure enriched the starting pool in a family of homologous sequences. High binding affinity (10nM) of one member of this family, aptamer 12.11, was demonstrated in a filter binding assay.",

keywords = "Aptamer, SELEX, VCAM-1",

author = "Fabien Chauveau and Youssef Aissouni and Jorg Hamm and Herve Boutin and Domenico Libri and Frederic Duconge and Bertrand Tavitian",

year = "2007",

month = nov,

day = "15",

doi = "10.1016/j.bmcl.2007.09.046",

language = "English",

volume = "17",

pages = "6119--6122",

journal = "Bioorganic and Medicinal Chemistry Letters",

issn = "1464-3405",

publisher = "Elsevier BV",

number = "22",

}

TY - JOUR

T1 - Binding of an aptamer to the N-terminal fragment of VCAM-1

AU - Chauveau, Fabien

AU - Aissouni, Youssef

AU - Hamm, Jorg

AU - Boutin, Herve

AU - Libri, Domenico

AU - Duconge, Frederic

AU - Tavitian, Bertrand

PY - 2007/11/15

Y1 - 2007/11/15

N2 - In vitro selection of 2'-fluoropyrimidine oligonucleotide aptamers was performed against the N-terminal two-domain fragment of mouse VCAM-1. The SELEX procedure enriched the starting pool in a family of homologous sequences. High binding affinity (10nM) of one member of this family, aptamer 12.11, was demonstrated in a filter binding assay.

AB - In vitro selection of 2'-fluoropyrimidine oligonucleotide aptamers was performed against the N-terminal two-domain fragment of mouse VCAM-1. The SELEX procedure enriched the starting pool in a family of homologous sequences. High binding affinity (10nM) of one member of this family, aptamer 12.11, was demonstrated in a filter binding assay.

KW - Aptamer

KW - SELEX

KW - VCAM-1

U2 - 10.1016/j.bmcl.2007.09.046

DO - 10.1016/j.bmcl.2007.09.046

M3 - Article

C2 - 17905582

SN - 1464-3405

VL - 17

SP - 6119

EP - 6122

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 22

ER -

Binding of an aptamer to the N-terminal fragment of VCAM-1

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this