Biocompatibility and osteogenic potential of human fetal femur-derived cells on surface selective laser sintered scaffolds

Janos M. Kanczler; Sayed Hadi Mirmalek-Sani; Neil A. Hanley; Alexander L. Ivanov; John J A Barry; Clare Upton; Kevin M. Shakesheff; Steven M. Howdle; Eugeuni N. Antonov; Victor N. Bagratashvili; Vladimir K. Popov; Richard O C Oreffo

doi:10.1016/j.actbio.2009.03.010

Biocompatibility and osteogenic potential of human fetal femur-derived cells on surface selective laser sintered scaffolds

Janos M. Kanczler, Sayed Hadi Mirmalek-Sani, Neil A. Hanley, Alexander L. Ivanov, John J A Barry, Clare Upton, Kevin M. Shakesheff, Steven M. Howdle, Eugeuni N. Antonov, Victor N. Bagratashvili, Vladimir K. Popov, Richard O C Oreffo

Research output: Contribution to journal › Article › peer-review

Abstract

For optimal bone regeneration, scaffolds need to fit anatomically into the requisite bone defects and, ideally, augment cell growth and differentiation. In this study we evaluated novel computationally designed surface selective laser sintering (SSLS) scaffolds for their biocompatibility as templates, in vitro and in vivo, for human fetal femur-derived cell viability, growth and osteogenesis. Fetal femur-derived cells were successfully cultured on SSLS-poly(d,l)-lactic acid (SSLS-PLA) scaffolds expressing alkaline phosphatase activity after 7 days. Cell proliferation, ingrowth, Alcian blue/Sirius red and type I collagen positive staining of matrix deposition were observed for fetal femur-derived cells cultured on SSLS-PLA scaffolds in vitro and in vivo. SSLS-PLA scaffolds and SSLS-PLA scaffolds seeded with fetal femur-derived cells implanted into a murine critical-sized femur segmental defect model aided the regeneration of the bone defect. SSLS techniques allow fabrication of biocompatible/biodegradable scaffolds, computationally designed to fit any defect, providing a template for cell osteogenesis in vitro and in vivo. © 2009 Acta Materialia Inc.

Original language	English
Pages (from-to)	2063-2071
Number of pages	8
Journal	Acta Biomaterialia
Volume	5
Issue number	6
DOIs	https://doi.org/10.1016/j.actbio.2009.03.010
Publication status	Published - Jul 2009

Keywords

Human fetal femur-derived cells
Osteogenesis
Surface selective laser sintering
Tissue engineering

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Kanczler, J. M., Mirmalek-Sani, S. H., Hanley, N. A., Ivanov, A. L., Barry, J. J. A., Upton, C., Shakesheff, K. M., Howdle, S. M., Antonov, E. N., Bagratashvili, V. N., Popov, V. K., & Oreffo, R. O. C. (2009). Biocompatibility and osteogenic potential of human fetal femur-derived cells on surface selective laser sintered scaffolds. Acta Biomaterialia, 5(6), 2063-2071. https://doi.org/10.1016/j.actbio.2009.03.010

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title = "Biocompatibility and osteogenic potential of human fetal femur-derived cells on surface selective laser sintered scaffolds",

abstract = "For optimal bone regeneration, scaffolds need to fit anatomically into the requisite bone defects and, ideally, augment cell growth and differentiation. In this study we evaluated novel computationally designed surface selective laser sintering (SSLS) scaffolds for their biocompatibility as templates, in vitro and in vivo, for human fetal femur-derived cell viability, growth and osteogenesis. Fetal femur-derived cells were successfully cultured on SSLS-poly(d,l)-lactic acid (SSLS-PLA) scaffolds expressing alkaline phosphatase activity after 7 days. Cell proliferation, ingrowth, Alcian blue/Sirius red and type I collagen positive staining of matrix deposition were observed for fetal femur-derived cells cultured on SSLS-PLA scaffolds in vitro and in vivo. SSLS-PLA scaffolds and SSLS-PLA scaffolds seeded with fetal femur-derived cells implanted into a murine critical-sized femur segmental defect model aided the regeneration of the bone defect. SSLS techniques allow fabrication of biocompatible/biodegradable scaffolds, computationally designed to fit any defect, providing a template for cell osteogenesis in vitro and in vivo. {\textcopyright} 2009 Acta Materialia Inc.",

keywords = "Human fetal femur-derived cells, Osteogenesis, Surface selective laser sintering, Tissue engineering",

author = "Kanczler, {Janos M.} and Mirmalek-Sani, {Sayed Hadi} and Hanley, {Neil A.} and Ivanov, {Alexander L.} and Barry, {John J A} and Clare Upton and Shakesheff, {Kevin M.} and Howdle, {Steven M.} and Antonov, {Eugeuni N.} and Bagratashvili, {Victor N.} and Popov, {Vladimir K.} and Oreffo, {Richard O C}",

year = "2009",

month = jul,

doi = "10.1016/j.actbio.2009.03.010",

language = "English",

volume = "5",

pages = "2063--2071",

journal = "Acta Biomaterialia",

issn = "1742-7061",

publisher = "Elsevier BV",

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Kanczler, JM, Mirmalek-Sani, SH, Hanley, NA, Ivanov, AL, Barry, JJA, Upton, C, Shakesheff, KM, Howdle, SM, Antonov, EN, Bagratashvili, VN, Popov, VK & Oreffo, ROC 2009, 'Biocompatibility and osteogenic potential of human fetal femur-derived cells on surface selective laser sintered scaffolds', Acta Biomaterialia, vol. 5, no. 6, pp. 2063-2071. https://doi.org/10.1016/j.actbio.2009.03.010

TY - JOUR

T1 - Biocompatibility and osteogenic potential of human fetal femur-derived cells on surface selective laser sintered scaffolds

AU - Kanczler, Janos M.

AU - Mirmalek-Sani, Sayed Hadi

AU - Hanley, Neil A.

AU - Ivanov, Alexander L.

AU - Barry, John J A

AU - Upton, Clare

AU - Shakesheff, Kevin M.

AU - Howdle, Steven M.

AU - Antonov, Eugeuni N.

AU - Bagratashvili, Victor N.

AU - Popov, Vladimir K.

AU - Oreffo, Richard O C

PY - 2009/7

Y1 - 2009/7

N2 - For optimal bone regeneration, scaffolds need to fit anatomically into the requisite bone defects and, ideally, augment cell growth and differentiation. In this study we evaluated novel computationally designed surface selective laser sintering (SSLS) scaffolds for their biocompatibility as templates, in vitro and in vivo, for human fetal femur-derived cell viability, growth and osteogenesis. Fetal femur-derived cells were successfully cultured on SSLS-poly(d,l)-lactic acid (SSLS-PLA) scaffolds expressing alkaline phosphatase activity after 7 days. Cell proliferation, ingrowth, Alcian blue/Sirius red and type I collagen positive staining of matrix deposition were observed for fetal femur-derived cells cultured on SSLS-PLA scaffolds in vitro and in vivo. SSLS-PLA scaffolds and SSLS-PLA scaffolds seeded with fetal femur-derived cells implanted into a murine critical-sized femur segmental defect model aided the regeneration of the bone defect. SSLS techniques allow fabrication of biocompatible/biodegradable scaffolds, computationally designed to fit any defect, providing a template for cell osteogenesis in vitro and in vivo. © 2009 Acta Materialia Inc.

AB - For optimal bone regeneration, scaffolds need to fit anatomically into the requisite bone defects and, ideally, augment cell growth and differentiation. In this study we evaluated novel computationally designed surface selective laser sintering (SSLS) scaffolds for their biocompatibility as templates, in vitro and in vivo, for human fetal femur-derived cell viability, growth and osteogenesis. Fetal femur-derived cells were successfully cultured on SSLS-poly(d,l)-lactic acid (SSLS-PLA) scaffolds expressing alkaline phosphatase activity after 7 days. Cell proliferation, ingrowth, Alcian blue/Sirius red and type I collagen positive staining of matrix deposition were observed for fetal femur-derived cells cultured on SSLS-PLA scaffolds in vitro and in vivo. SSLS-PLA scaffolds and SSLS-PLA scaffolds seeded with fetal femur-derived cells implanted into a murine critical-sized femur segmental defect model aided the regeneration of the bone defect. SSLS techniques allow fabrication of biocompatible/biodegradable scaffolds, computationally designed to fit any defect, providing a template for cell osteogenesis in vitro and in vivo. © 2009 Acta Materialia Inc.

KW - Human fetal femur-derived cells

KW - Osteogenesis

KW - Surface selective laser sintering

KW - Tissue engineering

U2 - 10.1016/j.actbio.2009.03.010

DO - 10.1016/j.actbio.2009.03.010

M3 - Article

SN - 1742-7061

VL - 5

SP - 2063

EP - 2071

JO - Acta Biomaterialia

JF - Acta Biomaterialia

IS - 6

ER -

Biocompatibility and osteogenic potential of human fetal femur-derived cells on surface selective laser sintered scaffolds

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Keywords

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