Biosynthesis of ethylene glycol in Escherichia coli

Huaiwei Liu, Kristine Rose M. Ramos, Kris Niño G. Valdehuesa, Grace M. Nisola, Won Keun Lee, Wook Jin Chung

Research output: Contribution to journalArticlepeer-review


Ethylene glycol (EG) is an important platform chemical with steadily expanding global demand. Its commercial production is currently limited to fossil resources; no biosynthesis route has been delineated. Herein, a biosynthesis route for EG production from d-xylose is reported. This route consists of four steps: d-xylose → d-xylonate → 2-dehydro-3-deoxy-d- pentonate → glycoaldehyde → EG. Respective enzymes, d-xylose dehydrogenase, d-xylonate dehydratase, 2-dehydro-3-deoxy-d-pentonate aldolase, and glycoaldehyde reductase, were assembled. The route was implemented in a metabolically engineered Escherichia coli, in which the d-xylose → d-xylulose reaction was prevented by disrupting the d-xylose isomerase gene. The most efficient construct produced 11.7 g L-1 of EG from 40.0 g L-1 of d-xylose. Glycolate is a carbon-competing by-product during EG production in E. coli; blockage of glycoaldehyde → glycolate reaction was also performed by disrupting the gene encoding aldehyde dehydrogenase, but from this approach, EG productivity was not improved but rather led to d-xylonate accumulation. To channel more carbon flux towards EG than the glycolate pathway, further systematic metabolic engineering and fermentation optimization studies are still required to improve EG productivity.

Original languageEnglish
Pages (from-to)3409-3417
Number of pages9
JournalApplied microbiology and biotechnology
Issue number8
Publication statusPublished - 1 Apr 2013
Externally publishedYes


  • Biosynthesis
  • d-Xylose
  • Escherichia coli
  • Ethylene glycol


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