Abstract
Macrophages (MPs) are present in many tissues and have been implicated in the excessive bone resorption seen in patients with skeletal disorders. Our previous studies showed that macrophage-like cells influenced osteoblasts (OB) in co-culture, as number and activity of osteoblasts were decreased in co-cultures compared with controls. Macrophages are probable precursors of osteoblasts which have been shown to be inhibited by bisphosphonates (BPs). Bisphosphonates also modulate macrophage and osteoblasts activity. This study investigated whether addition of bisphosphonates to co-cultures of osteoblast and macrophages could reduce or block the adverse effects of macrophages on osteoblasts. The results showed that, compared to controls, fewer osteoblasts were present over time in macrophage/osteoblast co-cultures (at day 12, 15.5×10 4 and 8.8×10 4; P
| Original language | English |
|---|---|
| Pages (from-to) | 554-563 |
| Number of pages | 9 |
| Journal | International Journal of Biochemistry and Cell Biology |
| Volume | 34 |
| Issue number | 5 |
| Publication status | Published - 2002 |
Keywords
- Bisphosphonates
- Cell culture
- Osteoblast and macrophages