Ca-activated chloride current and Na-Ca exchange have different timecourses during sarcoplasmic reticulum Ca release in ferret ventricular myocytes

A. W. Trafford; M. E. Díaz; D. A. Eisner

doi:10.1007/s004240050577

Ca-activated chloride current and Na-Ca exchange have different timecourses during sarcoplasmic reticulum Ca release in ferret ventricular myocytes

A. W. Trafford, M. E. Díaz, D. A. Eisner

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this work was to measure membrane currents activated by Ca release from the cardiac sarcoplasmic reticulum (s.r.). Intracellular Ca concentration ([Ca2+](i)) was measured using fluo-3 in patch clamped cells. Calcium release from the s.r. (whether occurring spontaneously or evoked by caffeine) produced changes of membrane current which could be separated into a Ca-activated Cl current which was inhibited by DIDS or Cl removal and a Na-Ca exchange current. Both these currents had different time courses from the measured Ca2+](i). Furthermore the Ca-activated Cl current decayed more quickly than did Na-Ca exchange. Possible explanations for the different kinetics of these two Ca-sensitive currents are discussed.

Original language	English
Pages (from-to)	743-745
Number of pages	2
Journal	Pflügers Archiv European Journal of Physiology
Volume	435
Issue number	5
DOIs	https://doi.org/10.1007/s004240050577
Publication status	Published - 1998

Keywords

Ca activated currents
Cardiac muscle
Subsarcolemmal Ca

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10.1007/s004240050577

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title = "Ca-activated chloride current and Na-Ca exchange have different timecourses during sarcoplasmic reticulum Ca release in ferret ventricular myocytes",

abstract = "The aim of this work was to measure membrane currents activated by Ca release from the cardiac sarcoplasmic reticulum (s.r.). Intracellular Ca concentration ([Ca2+](i)) was measured using fluo-3 in patch clamped cells. Calcium release from the s.r. (whether occurring spontaneously or evoked by caffeine) produced changes of membrane current which could be separated into a Ca-activated Cl current which was inhibited by DIDS or Cl removal and a Na-Ca exchange current. Both these currents had different time courses from the measured Ca2+](i). Furthermore the Ca-activated Cl current decayed more quickly than did Na-Ca exchange. Possible explanations for the different kinetics of these two Ca-sensitive currents are discussed.",

keywords = "Ca activated currents, Cardiac muscle, Subsarcolemmal Ca",

author = "Trafford, {A. W.} and D{\'i}az, {M. E.} and Eisner, {D. A.}",

year = "1998",

doi = "10.1007/s004240050577",

language = "English",

volume = "435",

pages = "743--745",

journal = "Pfl{\"u}gers Archiv European Journal of Physiology",

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TY - JOUR

T1 - Ca-activated chloride current and Na-Ca exchange have different timecourses during sarcoplasmic reticulum Ca release in ferret ventricular myocytes

AU - Trafford, A. W.

AU - Díaz, M. E.

AU - Eisner, D. A.

PY - 1998

Y1 - 1998

N2 - The aim of this work was to measure membrane currents activated by Ca release from the cardiac sarcoplasmic reticulum (s.r.). Intracellular Ca concentration ([Ca2+](i)) was measured using fluo-3 in patch clamped cells. Calcium release from the s.r. (whether occurring spontaneously or evoked by caffeine) produced changes of membrane current which could be separated into a Ca-activated Cl current which was inhibited by DIDS or Cl removal and a Na-Ca exchange current. Both these currents had different time courses from the measured Ca2+](i). Furthermore the Ca-activated Cl current decayed more quickly than did Na-Ca exchange. Possible explanations for the different kinetics of these two Ca-sensitive currents are discussed.

AB - The aim of this work was to measure membrane currents activated by Ca release from the cardiac sarcoplasmic reticulum (s.r.). Intracellular Ca concentration ([Ca2+](i)) was measured using fluo-3 in patch clamped cells. Calcium release from the s.r. (whether occurring spontaneously or evoked by caffeine) produced changes of membrane current which could be separated into a Ca-activated Cl current which was inhibited by DIDS or Cl removal and a Na-Ca exchange current. Both these currents had different time courses from the measured Ca2+](i). Furthermore the Ca-activated Cl current decayed more quickly than did Na-Ca exchange. Possible explanations for the different kinetics of these two Ca-sensitive currents are discussed.

KW - Ca activated currents

KW - Cardiac muscle

KW - Subsarcolemmal Ca

U2 - 10.1007/s004240050577

DO - 10.1007/s004240050577

M3 - Article

SN - 0031-6768

VL - 435

SP - 743

EP - 745

JO - Pflügers Archiv European Journal of Physiology

JF - Pflügers Archiv European Journal of Physiology

IS - 5

ER -

Ca-activated chloride current and Na-Ca exchange have different timecourses during sarcoplasmic reticulum Ca release in ferret ventricular myocytes

Abstract

Keywords

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