Calbindin-D9K gene expression in rat chorioallantoic placenta is not regulated by 1,25-dihydroxyvitamin D3.

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    The aim of this study was to investigate whether 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), the active metabolite of vitamin D, regulates the expression of rat placental calbindin-D9K mRNA. One group of rats (-D) was fed a vitamin D-deficient diet before and during pregnancy, whereas a second group (+D) was fed a vitamin D-replete diet over the same period. Animals were killed on d 21 of gestation, and plasma concentrations of 1,25(OH)2D3 for +D and -D animals were significantly (p <0.05) different (260 +/- 78 and 39 +/- 8 pM in maternal plasma and 122 +/- 39 and 42 +/- 10 pM in fetal plasma, respectively; mean +/- SE, n = 4-5). Vitamin D deficiency had no effect on placental weight, fetal weight, fetal ashed weight, fetal calcium accretion, or the maternofetal calcium gradient. Hybridization of RNA from maternal duodena (used as a positive control tissue) and placentas of +D and -D rats with a rat calbindin-D9K cDNA revealed a single 0.6-kb transcript in both tissues. The abundance of this transcript was markedly lower (p = 0.06) in the duodena of -D compared with +D rats (mean change -68 +/- 9%) but there was no difference between the placentas of the two groups (mean change +13 +/- 22%). These changes were significantly (p <0.05) different between the two tissues and the response of each tissue to vitamin D deficiency was significantly different (p <0.01). These data indicate that 1,25(OH)2D3 does not regulate the expression of calbindin-D9K mRNA in rat placenta.
    Original languageEnglish
    JournalPediatr Res
    Volume37( 6)
    Publication statusPublished - Jun 1995


    • drug effects: Allantois
    • Animals
    • blood: Calcifediol
    • blood: Calcitriol
    • blood: Calcium
    • genetics: Calcium-Binding Protein, Vitamin D-Dependent
    • drug effects: Chorion
    • Female
    • metabolism: Fetal Blood
    • drug effects: Gene Expression Regulation, Developmental
    • drug effects: Intestines
    • drug effects: Placenta
    • Pregnancy
    • genetics: Pregnancy Proteins
    • metabolism: RNA, Messenger
    • Rats
    • Rats, Wistar
    • blood: Vitamin D Deficiency


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