Calcium-induced calcium release activates contraction in intact cardiac cells

M. Valdeolmillos; S. C. O'Neill; G. L. Smith; D. A. Eisner

doi:10.1007/BF00581820

Calcium-induced calcium release activates contraction in intact cardiac cells

M. Valdeolmillos, S. C. O'Neill, G. L. Smith, D. A. Eisner

Research output: Contribution to journal › Article › peer-review

Abstract

The "caged" calcium chelator Nitr-5 was Incorporated into isolated rat ventricular myocytes. Brief illumination with ultra-violet light made the cell twitch. The light-induced twitch was inhibited by ryanodine (1-10 μM) suggesting that it resulted from calcium-induced release of calcium from the sarcoplasmic reticulum. Inhibition of the Ca current (Ni, 10 mM) abolished the electrically stimulated twitch but did not inhibit the light-induced twitch. These results provide direct evidence for the importance of Ca-induced Ca release in excitation-contraction coupling in the heart. © 1989 Springer-Verlag.

Original language	English
Pages (from-to)	676-678
Number of pages	2
Journal	Pflügers Archiv European Journal of Physiology
Volume	413
Issue number	6
DOIs	https://doi.org/10.1007/BF00581820
Publication status	Published - Apr 1989

Keywords

caged Ca
Cardiac myocyte
contraction
sarcoplasmic reticulum

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10.1007/BF00581820

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title = "Calcium-induced calcium release activates contraction in intact cardiac cells",

abstract = "The {"}caged{"} calcium chelator Nitr-5 was Incorporated into isolated rat ventricular myocytes. Brief illumination with ultra-violet light made the cell twitch. The light-induced twitch was inhibited by ryanodine (1-10 μM) suggesting that it resulted from calcium-induced release of calcium from the sarcoplasmic reticulum. Inhibition of the Ca current (Ni, 10 mM) abolished the electrically stimulated twitch but did not inhibit the light-induced twitch. These results provide direct evidence for the importance of Ca-induced Ca release in excitation-contraction coupling in the heart. {\textcopyright} 1989 Springer-Verlag.",

keywords = "caged Ca, Cardiac myocyte, contraction, sarcoplasmic reticulum",

author = "M. Valdeolmillos and O'Neill, {S. C.} and Smith, {G. L.} and Eisner, {D. A.}",

year = "1989",

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AU - Valdeolmillos, M.

AU - O'Neill, S. C.

AU - Smith, G. L.

AU - Eisner, D. A.

PY - 1989/4

Y1 - 1989/4

N2 - The "caged" calcium chelator Nitr-5 was Incorporated into isolated rat ventricular myocytes. Brief illumination with ultra-violet light made the cell twitch. The light-induced twitch was inhibited by ryanodine (1-10 μM) suggesting that it resulted from calcium-induced release of calcium from the sarcoplasmic reticulum. Inhibition of the Ca current (Ni, 10 mM) abolished the electrically stimulated twitch but did not inhibit the light-induced twitch. These results provide direct evidence for the importance of Ca-induced Ca release in excitation-contraction coupling in the heart. © 1989 Springer-Verlag.

AB - The "caged" calcium chelator Nitr-5 was Incorporated into isolated rat ventricular myocytes. Brief illumination with ultra-violet light made the cell twitch. The light-induced twitch was inhibited by ryanodine (1-10 μM) suggesting that it resulted from calcium-induced release of calcium from the sarcoplasmic reticulum. Inhibition of the Ca current (Ni, 10 mM) abolished the electrically stimulated twitch but did not inhibit the light-induced twitch. These results provide direct evidence for the importance of Ca-induced Ca release in excitation-contraction coupling in the heart. © 1989 Springer-Verlag.

KW - caged Ca

KW - Cardiac myocyte

KW - contraction

KW - sarcoplasmic reticulum

U2 - 10.1007/BF00581820

DO - 10.1007/BF00581820

M3 - Article

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EP - 678

JO - Pflügers Archiv European Journal of Physiology

JF - Pflügers Archiv European Journal of Physiology

IS - 6

ER -

Calcium-induced calcium release activates contraction in intact cardiac cells

Abstract

Keywords

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