Can you see the light: simple and affordable steps to exploit recent advances in fluorescence imaging.

While recent advances in imaging technology have had a dramatic impact upon biology, the technical challenges and baffling array of options have tended to restrict these technologies to a limited number of cogniscenti. This is particularly true for imaging microbes where low intensity signals have conspired with the need for considerable magnification to persent a formibible challenge. however, some straight forward changes to standard fluorescence microscopes can now satisfy this obsessive desire to maximize sensitivity and resolution.