Candida yeasts in chronic periodontitis tissues and subgingival microbial biofilms in vivo

A. Järvensivu, J. Hietanen, Rautemaa-Richardson Richardson, T. Sorsa, Malcolm Richardson

    Research output: Contribution to journalArticlepeer-review


    The frequency of Candida infection in periodontal tissues of chronic periodontitis (CP) patients and the extent of candidal penetration into gingival tissues was studied. Tissue specimens collected from 25 CP patients during periodontal flap operations of initial periodontal therapy were examined by immunohistochemistry using Candida albicans-specific antibodies. Sections were also stained with periodic acid-Schiff (PAS) and subgingival plaque samples from 17 patients were cultured. Immunoreactivity for Candida was present in four of the 25 CP specimens (16%). Only one yeast-positive specimen was found when PAS-staining was used (4%) and two yeast-positive specimens were found with plaque culture (8%). Hyphal formation seemed essential and candidal hyphae were found to extend into the periodontal connective tissue. The degree and type of inflammation adjacent to the hyphae varied from a site and a patient to another. The sensitivity of specific antibodies was superior to PAS-stain or plaque culture in detection of Candida in tissues.
    Original languageEnglish
    Pages (from-to)106-112
    Number of pages6
    JournalOral Diseases
    Issue number2
    Publication statusPublished - Mar 2004


    • Candida albicans
    • Chronic periodontitis
    • Immunohistochemistry
    • Monoclonal antibody 3H8
    • Polyclonal antibody 158


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