Capillary zone electrophoresis for the analysis of glycoforms of cellobiohydrolase

Ruchi Gupta; Sara J. Baldock; Peter R. Fielden; Bruce D. Grieve

doi:10.1016/j.chroma.2011.06.036

Capillary zone electrophoresis for the analysis of glycoforms of cellobiohydrolase

Ruchi Gupta, Sara J. Baldock, Peter R. Fielden, Bruce D. Grieve

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Abstract

Cellobiohydrolase (CBH) is an important enzyme for the conversion of lignocellulosic biomass to ethanol. This work separated the glycoforms of CBH possessing different numbers of neutral mannoses using capillary zone electrophoresis (CZE) in a 50. mM, pH 7.5 phosphate buffer. The method analysed CBH in an intact form using a polyacrylamide coated fused silica capillary without requiring additives or labelling of the enzyme. The migration time of the major peak was found to be 21.6 ± 0.1. min (n= 3) and the approach is suitable for testing of batch-to-batch consistency of CBH. Ease-of-use, automation and speed are the other benefits due to which the use of CZE for analysing glycoforms of CBH was concluded to be ideal. © 2011 Elsevier B.V.

Original language	English
Pages (from-to)	5362-5368
Number of pages	6
Journal	Journal of Chromatography A
Volume	1218
Issue number	31
DOIs	https://doi.org/10.1016/j.chroma.2011.06.036
Publication status	Published - 5 Aug 2011

Keywords

Biofuel
Capillary coating
Cellobiohydrolase
Electrophoresis
Glycoforms
Mannose

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Capillary zone electrophoresis for the analysis of glycoforms of cellobiohydrolase",

abstract = "Cellobiohydrolase (CBH) is an important enzyme for the conversion of lignocellulosic biomass to ethanol. This work separated the glycoforms of CBH possessing different numbers of neutral mannoses using capillary zone electrophoresis (CZE) in a 50. mM, pH 7.5 phosphate buffer. The method analysed CBH in an intact form using a polyacrylamide coated fused silica capillary without requiring additives or labelling of the enzyme. The migration time of the major peak was found to be 21.6 ± 0.1. min (n= 3) and the approach is suitable for testing of batch-to-batch consistency of CBH. Ease-of-use, automation and speed are the other benefits due to which the use of CZE for analysing glycoforms of CBH was concluded to be ideal. {\textcopyright} 2011 Elsevier B.V.",

keywords = "Biofuel, Capillary coating, Cellobiohydrolase, Electrophoresis, Glycoforms, Mannose",

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doi = "10.1016/j.chroma.2011.06.036",

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TY - JOUR

T1 - Capillary zone electrophoresis for the analysis of glycoforms of cellobiohydrolase

AU - Gupta, Ruchi

AU - Baldock, Sara J.

AU - Fielden, Peter R.

AU - Grieve, Bruce D.

PY - 2011/8/5

Y1 - 2011/8/5

N2 - Cellobiohydrolase (CBH) is an important enzyme for the conversion of lignocellulosic biomass to ethanol. This work separated the glycoforms of CBH possessing different numbers of neutral mannoses using capillary zone electrophoresis (CZE) in a 50. mM, pH 7.5 phosphate buffer. The method analysed CBH in an intact form using a polyacrylamide coated fused silica capillary without requiring additives or labelling of the enzyme. The migration time of the major peak was found to be 21.6 ± 0.1. min (n= 3) and the approach is suitable for testing of batch-to-batch consistency of CBH. Ease-of-use, automation and speed are the other benefits due to which the use of CZE for analysing glycoforms of CBH was concluded to be ideal. © 2011 Elsevier B.V.

AB - Cellobiohydrolase (CBH) is an important enzyme for the conversion of lignocellulosic biomass to ethanol. This work separated the glycoforms of CBH possessing different numbers of neutral mannoses using capillary zone electrophoresis (CZE) in a 50. mM, pH 7.5 phosphate buffer. The method analysed CBH in an intact form using a polyacrylamide coated fused silica capillary without requiring additives or labelling of the enzyme. The migration time of the major peak was found to be 21.6 ± 0.1. min (n= 3) and the approach is suitable for testing of batch-to-batch consistency of CBH. Ease-of-use, automation and speed are the other benefits due to which the use of CZE for analysing glycoforms of CBH was concluded to be ideal. © 2011 Elsevier B.V.

KW - Biofuel

KW - Capillary coating

KW - Cellobiohydrolase

KW - Electrophoresis

KW - Glycoforms

KW - Mannose

U2 - 10.1016/j.chroma.2011.06.036

DO - 10.1016/j.chroma.2011.06.036

M3 - Article

SN - 1873-3778

VL - 1218

SP - 5362

EP - 5368

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 31

ER -

Capillary zone electrophoresis for the analysis of glycoforms of cellobiohydrolase

Abstract

Keywords

UN SDGs

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