Carbohydrate antigen expression in murine embryonic stem cells and embryos. I. Lacto and neo-lacto determinants

D. G. Brown; V. N. Warren; P. Påhlsson; S. J. Kimber

doi:10.1007/BF00157810

Carbohydrate antigen expression in murine embryonic stem cells and embryos. I. Lacto and neo-lacto determinants

D. G. Brown, V. N. Warren, P. Påhlsson, S. J. Kimber

Research output: Contribution to journal › Article › peer-review

Abstract

A comparative study of lacto- and neo-lacto series carbohydrate antigens between the undifferentiated and differentiated derivatives of the embryonic stem (ES) cell line E14 and expression in the early embryo is reported. Antibodies to neo-lacto and lacto (type 1 and 2) precursor chains and blood group antigens such as H (types 1 and 2) A, B and Lewis (Le-a, Le-b, Le-x, Le-y) were examined. Backbone lacto- and neo-lacto structures were present on undifferentiated cells, as were terminal α-gal, SSEA-1, Le-y and low levels of Le-a. On differentiation, Le-x (SSEA-1 determinant) disappeared as has been found for embryonal carcinoma (EC) cells, and other determinants became restricted to cells of particular morphology. These observations will aid determination of the status and phenotypic stability of long-term embryonic stem-cell cultures. © 1993 Chapman & Hall.

Original language	English
Pages (from-to)	452-463
Number of pages	11
Journal	The Histochemical Journal
Volume	25
Issue number	6
DOIs	https://doi.org/10.1007/BF00157810
Publication status	Published - Jun 1993

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title = "Carbohydrate antigen expression in murine embryonic stem cells and embryos. I. Lacto and neo-lacto determinants",

abstract = "A comparative study of lacto- and neo-lacto series carbohydrate antigens between the undifferentiated and differentiated derivatives of the embryonic stem (ES) cell line E14 and expression in the early embryo is reported. Antibodies to neo-lacto and lacto (type 1 and 2) precursor chains and blood group antigens such as H (types 1 and 2) A, B and Lewis (Le-a, Le-b, Le-x, Le-y) were examined. Backbone lacto- and neo-lacto structures were present on undifferentiated cells, as were terminal α-gal, SSEA-1, Le-y and low levels of Le-a. On differentiation, Le-x (SSEA-1 determinant) disappeared as has been found for embryonal carcinoma (EC) cells, and other determinants became restricted to cells of particular morphology. These observations will aid determination of the status and phenotypic stability of long-term embryonic stem-cell cultures. {\textcopyright} 1993 Chapman & Hall.",

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T1 - Carbohydrate antigen expression in murine embryonic stem cells and embryos. I. Lacto and neo-lacto determinants

AU - Brown, D. G.

AU - Warren, V. N.

AU - Påhlsson, P.

AU - Kimber, S. J.

PY - 1993/6

Y1 - 1993/6

N2 - A comparative study of lacto- and neo-lacto series carbohydrate antigens between the undifferentiated and differentiated derivatives of the embryonic stem (ES) cell line E14 and expression in the early embryo is reported. Antibodies to neo-lacto and lacto (type 1 and 2) precursor chains and blood group antigens such as H (types 1 and 2) A, B and Lewis (Le-a, Le-b, Le-x, Le-y) were examined. Backbone lacto- and neo-lacto structures were present on undifferentiated cells, as were terminal α-gal, SSEA-1, Le-y and low levels of Le-a. On differentiation, Le-x (SSEA-1 determinant) disappeared as has been found for embryonal carcinoma (EC) cells, and other determinants became restricted to cells of particular morphology. These observations will aid determination of the status and phenotypic stability of long-term embryonic stem-cell cultures. © 1993 Chapman & Hall.

AB - A comparative study of lacto- and neo-lacto series carbohydrate antigens between the undifferentiated and differentiated derivatives of the embryonic stem (ES) cell line E14 and expression in the early embryo is reported. Antibodies to neo-lacto and lacto (type 1 and 2) precursor chains and blood group antigens such as H (types 1 and 2) A, B and Lewis (Le-a, Le-b, Le-x, Le-y) were examined. Backbone lacto- and neo-lacto structures were present on undifferentiated cells, as were terminal α-gal, SSEA-1, Le-y and low levels of Le-a. On differentiation, Le-x (SSEA-1 determinant) disappeared as has been found for embryonal carcinoma (EC) cells, and other determinants became restricted to cells of particular morphology. These observations will aid determination of the status and phenotypic stability of long-term embryonic stem-cell cultures. © 1993 Chapman & Hall.

U2 - 10.1007/BF00157810

DO - 10.1007/BF00157810

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JO - The Histochemical Journal

JF - The Histochemical Journal

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ER -

Carbohydrate antigen expression in murine embryonic stem cells and embryos. I. Lacto and neo-lacto determinants

Abstract

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