Carbon nanotubes in macrophages: Imaging and chemical analysis by X-ray fluorescence microscopy

Cyrill Bussy; Julien Cambedouzou; Sophie Lanone; Emilie Leccia; Vasile Heresanu; Mathieu Pinault; Martine Mayne-L'Hermite; Nathalie Brun; Claudie Mory; Marine Cotte; Jean Doucet; Jorge Boczkowski; Pascale Launois

doi:10.1021/nl800914m

Carbon nanotubes in macrophages: Imaging and chemical analysis by X-ray fluorescence microscopy

Cyrill Bussy, Julien Cambedouzou, Sophie Lanone, Emilie Leccia, Vasile Heresanu, Mathieu Pinault, Martine Mayne-L'Hermite, Nathalie Brun, Claudie Mory, Marine Cotte, Jean Doucet, Jorge Boczkowski (Corresponding), Pascale Launois (Corresponding)

Pharmacy

Research output: Contribution to journal › Article › peer-review

Abstract

X-ray fluorescence microscopy (microXRF) is applied for the first time to study macrophages exposed to unpurified and purified single-walled (SW) and multiwalled (MW) carbon nanotubes (CNT). Investigating chemical elemental distributions allows one to (i) image nanotube localization within a cell and (ii) detect chemical modification of the cell after CNT internalization. An excess of calcium is detected for cells exposed to unpurified SWCNT and MWCNT and related toxicological assays are discussed.

Original language	English
Pages (from-to)	2659-63
Number of pages	5
Journal	Nano Letters
Volume	8
Issue number	9
DOIs	https://doi.org/10.1021/nl800914m
Publication status	Published - 2 Aug 2008

Keywords

carbon nanotube
macrophage
Calcium signalling
x ray imaging

Research Beacons, Institutes and Platforms

Advanced materials

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10.1021/nl800914m

https://hal.archives-ouvertes.fr/hal-02925956/document

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title = "Carbon nanotubes in macrophages: Imaging and chemical analysis by X-ray fluorescence microscopy",

abstract = "X-ray fluorescence microscopy (microXRF) is applied for the first time to study macrophages exposed to unpurified and purified single-walled (SW) and multiwalled (MW) carbon nanotubes (CNT). Investigating chemical elemental distributions allows one to (i) image nanotube localization within a cell and (ii) detect chemical modification of the cell after CNT internalization. An excess of calcium is detected for cells exposed to unpurified SWCNT and MWCNT and related toxicological assays are discussed. ",

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year = "2008",

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doi = "10.1021/nl800914m",

language = "English",

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pages = "2659--63",

journal = "Nano Letters",

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TY - JOUR

T1 - Carbon nanotubes in macrophages

T2 - Imaging and chemical analysis by X-ray fluorescence microscopy

AU - Bussy, Cyrill

AU - Cambedouzou, Julien

AU - Lanone, Sophie

AU - Leccia, Emilie

AU - Heresanu, Vasile

AU - Pinault, Mathieu

AU - Mayne-L'Hermite, Martine

AU - Brun, Nathalie

AU - Mory, Claudie

AU - Cotte, Marine

AU - Doucet, Jean

A2 - Boczkowski, Jorge

A2 - Launois, Pascale

PY - 2008/8/2

Y1 - 2008/8/2

N2 - X-ray fluorescence microscopy (microXRF) is applied for the first time to study macrophages exposed to unpurified and purified single-walled (SW) and multiwalled (MW) carbon nanotubes (CNT). Investigating chemical elemental distributions allows one to (i) image nanotube localization within a cell and (ii) detect chemical modification of the cell after CNT internalization. An excess of calcium is detected for cells exposed to unpurified SWCNT and MWCNT and related toxicological assays are discussed.

AB - X-ray fluorescence microscopy (microXRF) is applied for the first time to study macrophages exposed to unpurified and purified single-walled (SW) and multiwalled (MW) carbon nanotubes (CNT). Investigating chemical elemental distributions allows one to (i) image nanotube localization within a cell and (ii) detect chemical modification of the cell after CNT internalization. An excess of calcium is detected for cells exposed to unpurified SWCNT and MWCNT and related toxicological assays are discussed.

KW - carbon nanotube

KW - macrophage

KW - Calcium signalling

KW - x ray imaging

U2 - 10.1021/nl800914m

DO - 10.1021/nl800914m

M3 - Article

SN - 1530-6992

VL - 8

SP - 2659

EP - 2663

JO - Nano Letters

JF - Nano Letters

IS - 9

ER -

Carbon nanotubes in macrophages: Imaging and chemical analysis by X-ray fluorescence microscopy

Abstract

Keywords

Research Beacons, Institutes and Platforms

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