CCR7-dependent trafficking of RORγ+ ILCs creates a unique microenvironment within mucosal draining lymph nodes

Emma Mackley; Stephanie Houston; Clare Marriott; Emily Halford; Beth Lucas; Vuk Cerovic; Kara Filbey; Rick Maizels; Matthew Hepworth; Gregory Sonnenberg; Simon Milling; David Withers

doi:10.1038/ncomms6862

CCR7-dependent trafficking of RORγ+ ILCs creates a unique microenvironment within mucosal draining lymph nodes

Emma Mackley, Stephanie Houston, Clare Marriott, Emily Halford, Beth Lucas, Vuk Cerovic, Kara Filbey, Rick Maizels, Matthew Hepworth, Gregory Sonnenberg, Simon Milling, David Withers

Division of Immunology, Immunity to Infection and Respiratory Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Presentation of peptide:MHCII by RORγ-expressing group 3 innate lymphoid cells (ILC3s), which are enriched within gut tissue, is required for control of CD4 T-cell responses to commensal bacteria. It is not known whether ILC populations migrate from their mucosal and peripheral sites to local draining secondary lymphoid tissues. Here we demonstrate that ILC3s reside within the interfollicular areas of mucosal draining lymph nodes, forming a distinct microenvironment not observed in peripheral lymph nodes. By photoconverting intestinal cells in Kaede mice we reveal constitutive trafficking of ILCs from the intestine to the draining mesenteric lymph nodes, which specifically for the LTi-like ILC3s was CCR7-dependent. Thus, ILC populations traffic to draining lymph nodes using different mechanisms.

Original language	English
Article number	5862
Journal	Nature Communications
Volume	6
DOIs	https://doi.org/10.1038/ncomms6862 https://doi.org/10.1038/ncomms6862
Publication status	Published - 9 Jan 2015

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Mackley, E., Houston, S., Marriott, C., Halford, E., Lucas, B., Cerovic, V., Filbey, K., Maizels, R., Hepworth, M., Sonnenberg, G., Milling, S., & Withers, D. (2015). CCR7-dependent trafficking of RORγ+ ILCs creates a unique microenvironment within mucosal draining lymph nodes. Nature Communications, 6, Article 5862. https://doi.org/10.1038/ncomms6862, https://doi.org/10.1038/ncomms6862

@article{01a6faeb1b5f492e9a05d0bd796e74d1,

title = "CCR7-dependent trafficking of RORγ+ ILCs creates a unique microenvironment within mucosal draining lymph nodes",

abstract = "Presentation of peptide:MHCII by RORγ-expressing group 3 innate lymphoid cells (ILC3s), which are enriched within gut tissue, is required for control of CD4 T-cell responses to commensal bacteria. It is not known whether ILC populations migrate from their mucosal and peripheral sites to local draining secondary lymphoid tissues. Here we demonstrate that ILC3s reside within the interfollicular areas of mucosal draining lymph nodes, forming a distinct microenvironment not observed in peripheral lymph nodes. By photoconverting intestinal cells in Kaede mice we reveal constitutive trafficking of ILCs from the intestine to the draining mesenteric lymph nodes, which specifically for the LTi-like ILC3s was CCR7-dependent. Thus, ILC populations traffic to draining lymph nodes using different mechanisms.",

author = "Emma Mackley and Stephanie Houston and Clare Marriott and Emily Halford and Beth Lucas and Vuk Cerovic and Kara Filbey and Rick Maizels and Matthew Hepworth and Gregory Sonnenberg and Simon Milling and David Withers",

note = "DP5 OD012116, NIH HHS, United StatesDP5OD012116, NIH HHS, United StatesMR/K021095/1, Medical Research Council, United Kingdom, Wellcome Trust, United Kingdom",

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Mackley, E, Houston, S, Marriott, C, Halford, E, Lucas, B, Cerovic, V, Filbey, K, Maizels, R, Hepworth, M, Sonnenberg, G, Milling, S & Withers, D 2015, 'CCR7-dependent trafficking of RORγ+ ILCs creates a unique microenvironment within mucosal draining lymph nodes', Nature Communications, vol. 6, 5862. https://doi.org/10.1038/ncomms6862, https://doi.org/10.1038/ncomms6862

TY - JOUR

T1 - CCR7-dependent trafficking of RORγ+ ILCs creates a unique microenvironment within mucosal draining lymph nodes

AU - Mackley, Emma

AU - Houston, Stephanie

AU - Marriott, Clare

AU - Halford, Emily

AU - Lucas, Beth

AU - Cerovic, Vuk

AU - Filbey, Kara

AU - Maizels, Rick

AU - Hepworth, Matthew

AU - Sonnenberg, Gregory

AU - Milling, Simon

AU - Withers, David

N1 - DP5 OD012116, NIH HHS, United StatesDP5OD012116, NIH HHS, United StatesMR/K021095/1, Medical Research Council, United Kingdom, Wellcome Trust, United Kingdom

PY - 2015/1/9

Y1 - 2015/1/9

N2 - Presentation of peptide:MHCII by RORγ-expressing group 3 innate lymphoid cells (ILC3s), which are enriched within gut tissue, is required for control of CD4 T-cell responses to commensal bacteria. It is not known whether ILC populations migrate from their mucosal and peripheral sites to local draining secondary lymphoid tissues. Here we demonstrate that ILC3s reside within the interfollicular areas of mucosal draining lymph nodes, forming a distinct microenvironment not observed in peripheral lymph nodes. By photoconverting intestinal cells in Kaede mice we reveal constitutive trafficking of ILCs from the intestine to the draining mesenteric lymph nodes, which specifically for the LTi-like ILC3s was CCR7-dependent. Thus, ILC populations traffic to draining lymph nodes using different mechanisms.

AB - Presentation of peptide:MHCII by RORγ-expressing group 3 innate lymphoid cells (ILC3s), which are enriched within gut tissue, is required for control of CD4 T-cell responses to commensal bacteria. It is not known whether ILC populations migrate from their mucosal and peripheral sites to local draining secondary lymphoid tissues. Here we demonstrate that ILC3s reside within the interfollicular areas of mucosal draining lymph nodes, forming a distinct microenvironment not observed in peripheral lymph nodes. By photoconverting intestinal cells in Kaede mice we reveal constitutive trafficking of ILCs from the intestine to the draining mesenteric lymph nodes, which specifically for the LTi-like ILC3s was CCR7-dependent. Thus, ILC populations traffic to draining lymph nodes using different mechanisms.

U2 - 10.1038/ncomms6862

DO - 10.1038/ncomms6862

M3 - Article

C2 - 25575242

SN - 2041-1723

VL - 6

JO - Nature Communications

JF - Nature Communications

M1 - 5862

ER -

CCR7-dependent trafficking of RORγ+ ILCs creates a unique microenvironment within mucosal draining lymph nodes

Abstract

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