CD1d-dependent macrophage-mediated clearance of Pseudomonas aeruginosa from lung

Edward E S Nieuwenhuis, Tetsuya Matsumoto, Mark Exley, Robbert A. Schleipman, Jonathan Glickman, Dan T. Bailey, Nadia Corazza, Sean P. Colgan, Andrew B. Onderdonk, Richard S. Blumberg

    Research output: Contribution to journalArticlepeer-review

    Abstract

    CD1d-restricted T cells are implicated as key players in host defense against various microbial infections. However, the mechanisms involved and the role they play, if any, at the mucosal surfaces where pathogenic infections are initiated is unknown. In a murine pneumonia model established by intranasal application of Pseudomonas aeruginosa, CD1d-/- mice showed markedly reduced pulmonary eradication of P. aeruginosa compared with wild-type mice; this was associated with significantly lower amounts of macrophage inflammatory protein-2 and reduced numbers of neutrophils within the bronchoalveolar lavage fluid. Corollarily, treatment of mice with α-galactosylceramide - a lipid that activates CD1d-restricted T cells - increased the amount of interferon-γ; this was associated with rapid pulmonary clearance through enhanced phagocytosis of P. aeruginosa by alveolar macrophages. These results reveal a crucial role played by CD1d-restricted T cells in regulating the antimicrobial immune functions of macrophages at the lung mucosal surface.
    Original languageEnglish
    Pages (from-to)588-593
    Number of pages5
    JournalNature Medicine
    Volume8
    Issue number6
    DOIs
    Publication statusPublished - 2002

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