C/EBPalpha initiates primitive myelopoiesis in pluripotent embryonic cells

Yaoyao Chen, Ricardo Da Costa, Nick R Love, Ximena Soto Rodriguez, Martin Roth, Roberto Paredes, Enrique Amaya

Research output: Contribution to journalArticlepeer-review


The molecular mechanisms that underlie the development of primitive myeloid cells in vertebrate embryos are not well understood. Here we characterize the role of cebpa during primitive myeloid cell development in Xenopus. We show that cebpa is one of the first known hematopoietic genes expressed in the embryo. Loss- and gain-of-function studies show that it is both necessary and sufficient for the development of functional myeloid cells. In addition, we show that cebpa misexpression leads to the precocious induction of myeloid cell markers in pluripotent prospective ectodermal cells, without the cells transitioning through a general mesodermal state. Finally, we use live imaging to show that cebpa-expressing cells exhibit many attributes of terminally differentiated myeloid cells, such as highly active migratory behavior, the ability to quickly and efficiently migrate toward wounds and phagocytose bacteria, and the ability to enter the circulation. Thus, C/EPBalpha is the first known single factor capable of initiating an entire myelopoiesis pathway in pluripotent cells in the embryo.
Original languageEnglish
Pages (from-to)40-48
Number of pages9
Issue number1
Publication statusPublished - 2 Jul 2009


  • myeloid cells
  • myelopoiesis
  • embryo
  • diagnostic imaging
  • CCAAT/enhancer-binding protein alpha


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