Cell biology: Calorie restriction promotes mitochondrial biogenesis by inducing the expression of eNOS

Enzo Nisoli, Cristina Tonello, Annalisa Cardile, Valeria Cozzi, Renata Bracale, Laura Tedesco, Sestina Falcone, Alessandra Valerio, Orazio Cantoni, Emilio Clementi, Salvador Moncada, Michele O. Carruba

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Calorie restriction extends life span in organisms ranging from yeast to mammals. Here, we report that calorie restriction for either 3 or 12 months induced endothelial nitric oxide synthase (eNOS) expression and 3′,5′-cyclic guanosine monophosphate formation in various tissues of male mice. This was accompanied by mitochondrial biogenesis, with increased oxygen consumption and adenosine triphosphate production, and an enhanced expression of sirtuin 1. These effects were strongly attenuated in eNOS null-mutant mice. Thus, nitric oxide plays a fundamental role in the processes induced by calorie restriction and may be involved in the extension of life span in mammals.
    Original languageEnglish
    Pages (from-to)314-317
    Number of pages3
    JournalScience
    Volume310
    Issue number5746
    DOIs
    Publication statusPublished - 14 Oct 2005

    Keywords

    • Adipose Tissue/metabolism
    • Animals
    • *Caloric Restriction
    • DNA, Mitochondrial/metabolism
    • Enzyme Induction
    • Female
    • GTP Phosphohydrolases/biosynthesis
    • Life Expectancy
    • Male
    • Mice
    • Mice, Inbred C57BL
    • Mitochondria/*physiology
    • Nitric Oxide/metabolism
    • Nitric Oxide Synthase/*biosynthesis/genetics
    • Nitric Oxide Synthase Type II
    • Nitric Oxide Synthase Type III
    • Oxygen Consumption
    • Protein Biosynthesis
    • Sirtuin 1
    • Sirtuins/biosynthesis

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