Cell therapy with human MSCs isolated from the umbilical cord Wharton jelly associated to a PVA membrane in the treatment of chronic skin wounds.

Jorge Ribeiro, Tiago Pereira, Irina Amorim, Ana Rita Caseiro, Maria A Lopes, Joana Lima, Andrea Gartner, José Domingos Santos, Paulo Jorge Da Silva bartolo, Jorge Manuel Rodrigues, Ana Colette Mauricio, Ana Lúcia Luís

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    Abstract

    The healing process of the skin is a dynamic procedure mediated through a complex feedback of growth factors secreted by a variety of cells types. Despite the most recent advances in wound healing management and surgical procedures, these techniques still fail up to 50%, so cellular therapies involving mesenchymal stem cells (MSCs) are nowadays a promising treatment of skin ulcers which are a cause of high morbidity. The MSCs modulate the inflammatory local response and induce cell replacing, by a paracrine mode of action, being an important cell therapy for the impaired wound healing. The local application of human MSCs (hMSCs) isolated from the umbilical cord Wharton's jelly together with a poly(vinyl alcohol) hydrogel (PVA) membrane, was tested to promote wound healing in two dogs that were referred for clinical examination at UPVET Hospital, showing non-healing large skin lesions by the standard treatments. The wounds were infiltrated with 1000 cells/µl hMSCs in a total volume of 100 µl per cm(2) of lesion area. A PVA membrane was applied to completely cover the wound to prevent its dehydration. Both animals after the treatment demonstrated a significant progress in skin regeneration with decreased extent of ulcerated areas confirmed by histological analysis. The use of Wharton's jelly MSCs associated with a PVA membrane showed promising clinical results for future application in the treatment of chronic wounds in companion animals and humans.
    Original languageEnglish
    Pages (from-to)979-987
    JournalInternational journal of medical sciences
    Volume11
    Issue number10
    DOIs
    Publication statusPublished - 11 Jul 2014

    Keywords

    • Chronic wounds
    • PVA hydrogel
    • Wharton's jelly
    • histology.
    • mesenchymal stem cells

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