Cellular methods in cartilage research: Primary human chondrocytes in culture and chondrogenesis in human bone marrow stem cells

Simon R. Tew; Alan D. Murdoch; Richard P. Rauchenberg; Timothy E. Hardingham

doi:10.1016/j.ymeth.2008.01.006

Cellular methods in cartilage research: Primary human chondrocytes in culture and chondrogenesis in human bone marrow stem cells

Simon R. Tew, Alan D. Murdoch, Richard P. Rauchenberg, Timothy E. Hardingham

Research output: Contribution to journal › Article › peer-review

Abstract

Work in our laboratory has focused on the in vitro culture of both human articular chondrocytes and human mesenchymal stem cells to understand what controls their ability to synthesise an appropriate cartilage-like extracellular matrix containing a predominantly collagen type II fibrillar network embedded in an aggrecan-rich ECM. This review focuses on the methodologies that we have found to be successful with cartilage and bone marrow sources of human cells and comments on the many factors which may enable improved phenotypic performance once the cells are in a fully chondrogenic environment. © 2008 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	2-9
Number of pages	7
Journal	Methods
Volume	45
Issue number	1
DOIs	https://doi.org/10.1016/j.ymeth.2008.01.006
Publication status	Published - May 2008

Keywords

Cartilage
Chondrocytes
Chondrogenesis
SOX9
Stem cells
Tissue engineering
Transwell

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abstract = "Work in our laboratory has focused on the in vitro culture of both human articular chondrocytes and human mesenchymal stem cells to understand what controls their ability to synthesise an appropriate cartilage-like extracellular matrix containing a predominantly collagen type II fibrillar network embedded in an aggrecan-rich ECM. This review focuses on the methodologies that we have found to be successful with cartilage and bone marrow sources of human cells and comments on the many factors which may enable improved phenotypic performance once the cells are in a fully chondrogenic environment. {\textcopyright} 2008 Elsevier Inc. All rights reserved.",

keywords = "Cartilage, Chondrocytes, Chondrogenesis, SOX9, Stem cells, Tissue engineering, Transwell",

author = "Tew, {Simon R.} and Murdoch, {Alan D.} and Rauchenberg, {Richard P.} and Hardingham, {Timothy E.}",

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AU - Murdoch, Alan D.

AU - Rauchenberg, Richard P.

AU - Hardingham, Timothy E.

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AB - Work in our laboratory has focused on the in vitro culture of both human articular chondrocytes and human mesenchymal stem cells to understand what controls their ability to synthesise an appropriate cartilage-like extracellular matrix containing a predominantly collagen type II fibrillar network embedded in an aggrecan-rich ECM. This review focuses on the methodologies that we have found to be successful with cartilage and bone marrow sources of human cells and comments on the many factors which may enable improved phenotypic performance once the cells are in a fully chondrogenic environment. © 2008 Elsevier Inc. All rights reserved.

KW - Cartilage

KW - Chondrocytes

KW - Chondrogenesis

KW - SOX9

KW - Stem cells

KW - Tissue engineering

KW - Transwell

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JO - Methods

JF - Methods

IS - 1

ER -

Cellular methods in cartilage research: Primary human chondrocytes in culture and chondrogenesis in human bone marrow stem cells

Abstract

Keywords

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