Central metabolism is tuned to the availability of oxygen in developing melon fruit

Kentaro Mori, Bertrand P. Beauvoit, Benoît Biais, Maxime Chabane, J. William Allwood, Catherine Deborde, Mickaël Maucourt, Royston Goodacre, Cécile Cabasson, Annick Moing, Dominique Rolin, Yves Gibon

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Respiration of bulky plant organs such as fleshy fruits depends on oxygen (O2) availability and often decreases with O2 concentration to avoid anoxia, but the relationship between O2 diffusional resistance and metabolic adjustments remains unclear. Melon fruit (Cucumis melo L.) was used to study relationships between O2 availability and metabolism in fleshy fruits. Enzyme activities, primary metabolites and O2 partial pressure were quantified from the periphery to the inner fruit mesocarp, at three stages of development. Hypoxia was gradually established during fruit development, but there was no strong oxygen gradient between the outer- and the inner mesocarp. These trends were confirmed by a mathematical modeling approach combining O2 diffusion equations and O2 demand estimates of the mesocarp tissue. A multivariate analysis of metabolites, enzyme activities, O2 demand and concentration reveals that metabolite gradients and enzyme capacities observed in melon fruits reflect continuous metabolic adjustments thus ensuring a timely maturation of the mesocarp. The present results suggest that the metabolic adjustments, especially the tuning of the capacity of cytochrome c oxidase (COX) to O2-availability that occurs during growth development, contribute to optimizing the O2-demand and avoiding the establishment of an O2 gradient within the flesh.

    Original languageEnglish
    Article number594
    JournalFrontiers in Plant Science
    Volume10
    Early online date15 May 2019
    DOIs
    Publication statusPublished - 2019

    Keywords

    • Cucumis melo
    • Cytochrome c oxidase
    • Fruit
    • Hypoxia
    • Metabolism
    • Modeling

    Fingerprint

    Dive into the research topics of 'Central metabolism is tuned to the availability of oxygen in developing melon fruit'. Together they form a unique fingerprint.

    Cite this