Changes in vanilloid receptor 1 (TRPV1) expression following lingual nerve injury

James E. Biggs, Julian M. Yates, Alison R. Loescher, Nick M. Clayton, Fiona M. Boissonade, Peter P. Robinson

    Research output: Contribution to journalArticlepeer-review

    Abstract

    We have investigated a possible role for vanilloid receptor 1 (TRPV1), a transducer of noxious stimuli, in the development of neuropathic pain following injury to a peripheral branch of the trigeminal nerve. In nine adult ferrets the left lingual nerve was sectioned and recovery permitted for 3 days, 3 weeks or 3 months (3 ferrets per group). A retrograde tracer, fluorogold, was injected into the damaged nerve to identify associated cell bodies in the trigeminal ganglion. Three further ferrets, receiving only tracer injection, served as uninjured controls. Indirect immunofluorescence for TRPV1 and image analysis was used to quantify the percentage area of staining (PAS) of TRPV1 in the left and right lingual nerves. Additionally, the proportion of fluorogold positive and fluorogold negative cells expressing TRPV1 in the ganglion was determined. TRPV1 expression increased significantly at the injury site of damaged nerves 3 days after injury and this was matched by a reduction in the proportion of fluorogold positive cells expressing TRPV1 in the ganglion. At 3 weeks TRPV1 expression at the injury site was still high, while in the ganglion was significantly greater than in the controls. In the 3-month recovery group TRPV1 expression in both nerve fibres and ganglion cells, was not significantly different from controls and there were no changes in expression in the fluorogold negative cells in the ganglion at any time point studied. These data suggest that after injury there is an increase in the axonal transport of TRPV1 from the cell bodies to the damaged axons and this is followed by an increase in synthesis in the ganglion. These changes in expression may be involved in development of sensory disturbances or dysaesthesia after injury. © 2006 European Federation of Chapters of the International Association for the Study of Pain.
    Original languageEnglish
    Pages (from-to)192-201
    Number of pages9
    JournalEuropean Journal of Pain
    Volume11
    Issue number2
    DOIs
    Publication statusPublished - Feb 2007

    Keywords

    • Ectopic activity
    • Lingual nerve
    • Neuropathic pain
    • Trigeminal ganglion
    • TRPV1

    Fingerprint

    Dive into the research topics of 'Changes in vanilloid receptor 1 (TRPV1) expression following lingual nerve injury'. Together they form a unique fingerprint.

    Cite this