Chapter 10. Photoactivation and Dissociation in Biomolecular Mass Spectrometry

Rachelle Black, Perdita E. Barran, Lennart A. I. Ramakers

Research output: Chapter in Book/Conference proceedingChapterpeer-review

Abstract

In this chapter, we highlight recent developments using laser irradiation to probe ions in mass spectrometers. We will primarily cover the structural characterisation of biomolecules. This chapter will introduce the principles behind laser irradiation and describe some of the common instrument configurations, with an emphasis on benchtop laser systems coupled to instruments in laboratories. We have ordered the narrative in this chapter according to what has been achieved with three different irradiation wavelengths namely UV, visible and infrared. Instrumentation developed to permit laser irradiation along with mass spectrometry is described in each section along with some examples of recent applications primarily focused on the analysis of peptides and proteins.
Original languageEnglish
Title of host publicationAdvanced Fragmentation Methods in Biomolecular Mass Spectrometry: Probing Primary and Higher Order Structure with Electrons, Photons and Surfaces
PublisherRoyal Society of Chemistry
Pages235-280
DOIs
Publication statusPublished - 2021

Publication series

NameAdvanced Fragmentation Methods in Biomolecular Mass Spectrometry
ISSN (Print)2045-7553

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