Chapter 18 Visualization of Desmosomes in the Electron Microscope

Anthea Scothern; David Garrod

doi:10.1016/S0091-679X(08)00418-4

Chapter 18 Visualization of Desmosomes in the Electron Microscope

Anthea Scothern, David Garrod

Research output: Contribution to journal › Article › peer-review

Abstract

Desmosomes are intercellular junctions found in epithelia and some other tissues. Their primary function is strong cell-cell adhesion. They also link the intermediate filament (IF) cytoskeletons between cells and have roles in cell signaling, tissue morphogenesis, and wound repair. Because of their size (0.2-0.5 μm), details of their ultrastructure can only be resolved at the electron microscope (EM) level. Desmosomes have been visualized using a variety of ultrastructural techniques including lanthanum infiltration, freeze-fracture, electron tomography, cryo-electron microscopy and immunogold labeling. This chapter describes protocols for conventional transmission electron microscopy and for immunogold labeling of ultrathin cryosections. We also discuss the statistical analysis of immunogold particle distribution for low resolution molecular mapping. © 2008 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	347-366
Number of pages	19
Journal	Methods in Cell Biology
Volume	88
DOIs	https://doi.org/10.1016/S0091-679X(08)00418-4
Publication status	Published - 2008

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title = "Chapter 18 Visualization of Desmosomes in the Electron Microscope",

abstract = "Desmosomes are intercellular junctions found in epithelia and some other tissues. Their primary function is strong cell-cell adhesion. They also link the intermediate filament (IF) cytoskeletons between cells and have roles in cell signaling, tissue morphogenesis, and wound repair. Because of their size (0.2-0.5 μm), details of their ultrastructure can only be resolved at the electron microscope (EM) level. Desmosomes have been visualized using a variety of ultrastructural techniques including lanthanum infiltration, freeze-fracture, electron tomography, cryo-electron microscopy and immunogold labeling. This chapter describes protocols for conventional transmission electron microscopy and for immunogold labeling of ultrathin cryosections. We also discuss the statistical analysis of immunogold particle distribution for low resolution molecular mapping. {\textcopyright} 2008 Elsevier Inc. All rights reserved.",

author = "Anthea Scothern and David Garrod",

year = "2008",

doi = "10.1016/S0091-679X(08)00418-4",

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volume = "88",

pages = "347--366",

journal = "Methods in Cell Biology",

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AB - Desmosomes are intercellular junctions found in epithelia and some other tissues. Their primary function is strong cell-cell adhesion. They also link the intermediate filament (IF) cytoskeletons between cells and have roles in cell signaling, tissue morphogenesis, and wound repair. Because of their size (0.2-0.5 μm), details of their ultrastructure can only be resolved at the electron microscope (EM) level. Desmosomes have been visualized using a variety of ultrastructural techniques including lanthanum infiltration, freeze-fracture, electron tomography, cryo-electron microscopy and immunogold labeling. This chapter describes protocols for conventional transmission electron microscopy and for immunogold labeling of ultrathin cryosections. We also discuss the statistical analysis of immunogold particle distribution for low resolution molecular mapping. © 2008 Elsevier Inc. All rights reserved.

U2 - 10.1016/S0091-679X(08)00418-4

DO - 10.1016/S0091-679X(08)00418-4

M3 - Article

VL - 88

SP - 347

EP - 366

JO - Methods in Cell Biology

JF - Methods in Cell Biology

ER -

Chapter 18 Visualization of Desmosomes in the Electron Microscope

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