Characterisation of cell-penetrating peptide-mediated peptide delivery

Simon W. Jones, Richard Christison, Ken Bundell, Catherine J. Voyce, Sarah M V Brockbank, Peter Newham, Mark A. Lindsay

    Research output: Contribution to journalArticlepeer-review


    Cell-penetrating peptides such as antennapedia, TAT, transportan and polyarginine have been extensively employed for in vitro and in vivo delivery of biologically active peptides. However, little is known of the relative efficacy, toxicity and uptake mechanism of individual protein transduction domain-peptide conjugates, factors that will be critical in determining the most effective sequence. In the present study, we show by FACS analysis that unconjugated antennapedia, TAT, transportan and polyarginine demonstrate similar kinetic uptake profiles, being maximal at 1-3 h and independent of cell type (HeLa, A549 and CHO cell lines). A comparison of the magnitude of uptake of cell-penetrating peptide conjugates demonstrated that polyarginine= transportan>antennapedia> TAT. However, examination of cellular toxicity showed that antennapedia
    Original languageEnglish
    Pages (from-to)1093-1102
    Number of pages9
    JournalBritish Journal of Pharmacology
    Issue number8
    Publication statusPublished - 2005


    • Antennapedia
    • Polyarginine
    • Protein transduction domain
    • TAT
    • Transportan


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