Characterisation of cell-penetrating peptide-mediated peptide delivery

Simon W. Jones; Richard Christison; Ken Bundell; Catherine J. Voyce; Sarah M V Brockbank; Peter Newham; Mark A. Lindsay

doi:10.1038/sj.bjp.0706279

Characterisation of cell-penetrating peptide-mediated peptide delivery

Simon W. Jones, Richard Christison, Ken Bundell, Catherine J. Voyce, Sarah M V Brockbank, Peter Newham, Mark A. Lindsay

Research output: Contribution to journal › Article › peer-review

Abstract

Cell-penetrating peptides such as antennapedia, TAT, transportan and polyarginine have been extensively employed for in vitro and in vivo delivery of biologically active peptides. However, little is known of the relative efficacy, toxicity and uptake mechanism of individual protein transduction domain-peptide conjugates, factors that will be critical in determining the most effective sequence. In the present study, we show by FACS analysis that unconjugated antennapedia, TAT, transportan and polyarginine demonstrate similar kinetic uptake profiles, being maximal at 1-3 h and independent of cell type (HeLa, A549 and CHO cell lines). A comparison of the magnitude of uptake of cell-penetrating peptide conjugates demonstrated that polyarginine= transportan>antennapedia> TAT. However, examination of cellular toxicity showed that antennapedia

Original language	English
Pages (from-to)	1093-1102
Number of pages	9
Journal	British Journal of Pharmacology
Volume	145
Issue number	8
DOIs	https://doi.org/10.1038/sj.bjp.0706279
Publication status	Published - 2005

Keywords

Antennapedia
Polyarginine
Protein transduction domain
TAT
Transportan

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/sj.bjp.0706279

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title = "Characterisation of cell-penetrating peptide-mediated peptide delivery",

abstract = "Cell-penetrating peptides such as antennapedia, TAT, transportan and polyarginine have been extensively employed for in vitro and in vivo delivery of biologically active peptides. However, little is known of the relative efficacy, toxicity and uptake mechanism of individual protein transduction domain-peptide conjugates, factors that will be critical in determining the most effective sequence. In the present study, we show by FACS analysis that unconjugated antennapedia, TAT, transportan and polyarginine demonstrate similar kinetic uptake profiles, being maximal at 1-3 h and independent of cell type (HeLa, A549 and CHO cell lines). A comparison of the magnitude of uptake of cell-penetrating peptide conjugates demonstrated that polyarginine= transportan>antennapedia> TAT. However, examination of cellular toxicity showed that antennapedia",

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doi = "10.1038/sj.bjp.0706279",

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T1 - Characterisation of cell-penetrating peptide-mediated peptide delivery

AU - Jones, Simon W.

AU - Christison, Richard

AU - Bundell, Ken

AU - Voyce, Catherine J.

AU - Brockbank, Sarah M V

AU - Newham, Peter

AU - Lindsay, Mark A.

PY - 2005

Y1 - 2005

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AB - Cell-penetrating peptides such as antennapedia, TAT, transportan and polyarginine have been extensively employed for in vitro and in vivo delivery of biologically active peptides. However, little is known of the relative efficacy, toxicity and uptake mechanism of individual protein transduction domain-peptide conjugates, factors that will be critical in determining the most effective sequence. In the present study, we show by FACS analysis that unconjugated antennapedia, TAT, transportan and polyarginine demonstrate similar kinetic uptake profiles, being maximal at 1-3 h and independent of cell type (HeLa, A549 and CHO cell lines). A comparison of the magnitude of uptake of cell-penetrating peptide conjugates demonstrated that polyarginine= transportan>antennapedia> TAT. However, examination of cellular toxicity showed that antennapedia

KW - Antennapedia

KW - Polyarginine

KW - Protein transduction domain

KW - TAT

KW - Transportan

U2 - 10.1038/sj.bjp.0706279

DO - 10.1038/sj.bjp.0706279

M3 - Article

SN - 0007-1188

VL - 145

SP - 1093

EP - 1102

JO - British Journal of Pharmacology

JF - British Journal of Pharmacology

IS - 8

ER -

Characterisation of cell-penetrating peptide-mediated peptide delivery

Abstract

Keywords

UN SDGs

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