Abstract
Cell-penetrating peptides such as antennapedia, TAT, transportan and polyarginine have been extensively employed for in vitro and in vivo delivery of biologically active peptides. However, little is known of the relative efficacy, toxicity and uptake mechanism of individual protein transduction domain-peptide conjugates, factors that will be critical in determining the most effective sequence. In the present study, we show by FACS analysis that unconjugated antennapedia, TAT, transportan and polyarginine demonstrate similar kinetic uptake profiles, being maximal at 1-3 h and independent of cell type (HeLa, A549 and CHO cell lines). A comparison of the magnitude of uptake of cell-penetrating peptide conjugates demonstrated that polyarginine= transportan>antennapedia> TAT. However, examination of cellular toxicity showed that antennapedia
Original language | English |
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Pages (from-to) | 1093-1102 |
Number of pages | 9 |
Journal | British Journal of Pharmacology |
Volume | 145 |
Issue number | 8 |
DOIs | |
Publication status | Published - 2005 |
Keywords
- Antennapedia
- Polyarginine
- Protein transduction domain
- TAT
- Transportan