Characterisation of leukocytes in a human skin blister model of acute inflammation and resolution

William Jenner, Madhur Motwani, Kristin Veighey, Justine Newson, Tatsiana Audzevich, Anna Nicolaou, Sharon Murphy, Raymond MacAllister, Derek W. Gilroy

    Research output: Contribution to journalArticlepeer-review


    There is an increasing need to understand the leukocytes and soluble mediators that drive acute inflammation and bring about its resolution in humans. We therefore carried out an extensive characterisation of the cantharidin skin blister model in healthy male volunteers. A novel fluorescence staining protocol was designed and implemented, which facilitated the identification of cell populations by flow cytometry. We observed that at the onset phase, 24 h after blister formation, the predominant cells were CD16 hi/CD66b+ PMNs followed by HLA-DR+/CD14 + monocytes/macrophages, CD11c+ and CD141+ dendritic cells as well as Siglec-8+ eosinophils. CD3+ T cells, CD19+ B cells and CD56+ NK cells were also present, but in comparatively fewer numbers. During resolution, 72 h following blister induction, numbers of PMNs declined whilst the numbers of monocyte/macrophages remain unchanged, though they upregulated expression of CD16 and CD163. In contrast, the overall numbers of dendritic cells and Siglec-8+ eosinophils increased. Post hoc analysis of these data revealed that of the inflammatory cytokines measured, TNF-α but not IL-1β or IL-8 correlated with increased PMN numbers at the onset. Volunteers with the greatest PMN infiltration at onset displayed the fastest clearance rates for these cells at resolution. Collectively, these data provide insight into the cells that occupy acute resolving blister in humans, the soluble mediators that may control their influx as well as the phenotype of mononuclear phagocytes that predominate the resolution phase. Further use of this model will improve our understanding of the evolution and resolution of inflammation in humans, how defects in these over-lapping pathways may contribute to the variability in disease longevity/chronicity, and lends itself to the screen of putative anti-inflammatory or pro-resolution therapies. © 2014 Jenner et al.
    Original languageEnglish
    Article numbere89375
    JournalPLoS ONE
    Issue number3
    Publication statusPublished - 6 Mar 2014


    Dive into the research topics of 'Characterisation of leukocytes in a human skin blister model of acute inflammation and resolution'. Together they form a unique fingerprint.

    Cite this