Characterisation of long term cat placental explant cultures: Uptake of taurine by system β

EE Champion; SJ Mann; JD Glazier; CJ Jones; JM Rawlings; CP Sibley; SL Greenwood

doi:10.1016/j.placenta.2004.07.010

Characterisation of long term cat placental explant cultures: Uptake of taurine by system β

EE Champion, SJ Mann, JD Glazier, CJ Jones, JM Rawlings, CP Sibley, SL Greenwood

Research output: Contribution to journal › Article › peer-review

Abstract

Dietary taurine is essential for cats and deficiency during pregnancy may lead to abortion, growth restriction or impaired neurological function of kittens. We previously described Na+- and Cl--dependent taurine transport by system β in fragments of freshly isolated cat placenta [Champion EE, Bailey SJ, Glazier JD, Jones CJP, Mann SJ, Rawlings JM, et al. Taurine uptake into cat placental tissue fragments. Placenta 2001;22:A.42]. Here we evaluate long term culture of cat placental explants as a model for the future study of chronic nutrient regulation of amino acid transport in this species. The cat placental explants displayed (i) Na+ -dependent [3H]taurine uptake and (ii) taurine transporter protein on day 7 of culture, as observed in fresh cat placental fragments. The explants had preserved the ability to secrete PGF2α hormone until day 11 of culture and remained morphologically largely intact until day 7 of culture. This model of placental explant culture will provide an important in vitro method for the study of chronic regulation of amino acid transport in the cat. © 2004 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	608-616
Number of pages	8
Journal	Placenta
Volume	26
Issue number	8-9
DOIs	https://doi.org/10.1016/j.placenta.2004.07.010 https://doi.org/10.1016/j.placenta.2004.07.010
Publication status	Published - Sept 2005

Keywords

Amino acid
Endotheliochorial
PGF2α
Taurine

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@article{b377440d02d545f38cdef61d9953d775,

title = "Characterisation of long term cat placental explant cultures: Uptake of taurine by system β",

abstract = "Dietary taurine is essential for cats and deficiency during pregnancy may lead to abortion, growth restriction or impaired neurological function of kittens. We previously described Na+- and Cl--dependent taurine transport by system β in fragments of freshly isolated cat placenta [Champion EE, Bailey SJ, Glazier JD, Jones CJP, Mann SJ, Rawlings JM, et al. Taurine uptake into cat placental tissue fragments. Placenta 2001;22:A.42]. Here we evaluate long term culture of cat placental explants as a model for the future study of chronic nutrient regulation of amino acid transport in this species. The cat placental explants displayed (i) Na+ -dependent [3H]taurine uptake and (ii) taurine transporter protein on day 7 of culture, as observed in fresh cat placental fragments. The explants had preserved the ability to secrete PGF2α hormone until day 11 of culture and remained morphologically largely intact until day 7 of culture. This model of placental explant culture will provide an important in vitro method for the study of chronic regulation of amino acid transport in the cat. {\textcopyright} 2004 Elsevier Ltd. All rights reserved.",

keywords = "Amino acid, Endotheliochorial, PGF2α, Taurine",

author = "EE Champion and SJ Mann and JD Glazier and CJ Jones and JM Rawlings and CP Sibley and SL Greenwood",

year = "2005",

month = sep,

doi = "10.1016/j.placenta.2004.07.010",

language = "English",

volume = "26",

pages = "608--616",

journal = "Placenta",

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T1 - Characterisation of long term cat placental explant cultures

T2 - Uptake of taurine by system β

AU - Champion, EE

AU - Mann, SJ

AU - Glazier, JD

AU - Jones, CJ

AU - Rawlings, JM

AU - Sibley, CP

AU - Greenwood, SL

PY - 2005/9

Y1 - 2005/9

N2 - Dietary taurine is essential for cats and deficiency during pregnancy may lead to abortion, growth restriction or impaired neurological function of kittens. We previously described Na+- and Cl--dependent taurine transport by system β in fragments of freshly isolated cat placenta [Champion EE, Bailey SJ, Glazier JD, Jones CJP, Mann SJ, Rawlings JM, et al. Taurine uptake into cat placental tissue fragments. Placenta 2001;22:A.42]. Here we evaluate long term culture of cat placental explants as a model for the future study of chronic nutrient regulation of amino acid transport in this species. The cat placental explants displayed (i) Na+ -dependent [3H]taurine uptake and (ii) taurine transporter protein on day 7 of culture, as observed in fresh cat placental fragments. The explants had preserved the ability to secrete PGF2α hormone until day 11 of culture and remained morphologically largely intact until day 7 of culture. This model of placental explant culture will provide an important in vitro method for the study of chronic regulation of amino acid transport in the cat. © 2004 Elsevier Ltd. All rights reserved.

AB - Dietary taurine is essential for cats and deficiency during pregnancy may lead to abortion, growth restriction or impaired neurological function of kittens. We previously described Na+- and Cl--dependent taurine transport by system β in fragments of freshly isolated cat placenta [Champion EE, Bailey SJ, Glazier JD, Jones CJP, Mann SJ, Rawlings JM, et al. Taurine uptake into cat placental tissue fragments. Placenta 2001;22:A.42]. Here we evaluate long term culture of cat placental explants as a model for the future study of chronic nutrient regulation of amino acid transport in this species. The cat placental explants displayed (i) Na+ -dependent [3H]taurine uptake and (ii) taurine transporter protein on day 7 of culture, as observed in fresh cat placental fragments. The explants had preserved the ability to secrete PGF2α hormone until day 11 of culture and remained morphologically largely intact until day 7 of culture. This model of placental explant culture will provide an important in vitro method for the study of chronic regulation of amino acid transport in the cat. © 2004 Elsevier Ltd. All rights reserved.

KW - Amino acid

KW - Endotheliochorial

KW - PGF2α

KW - Taurine

UR - http://europepmc.org/abstract/med/16085040

U2 - 10.1016/j.placenta.2004.07.010

DO - 10.1016/j.placenta.2004.07.010

M3 - Article

C2 - 16085040

SN - 0143-4004

VL - 26

SP - 608

EP - 616

JO - Placenta

JF - Placenta

IS - 8-9

ER -

Characterisation of long term cat placental explant cultures: Uptake of taurine by system β

Abstract

Keywords

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