Characterization of a conditional interleukin-1 receptor 1 mouse mutant using the Cre/LoxP system

Wesam Abdulaal, Catherine Walker, Ryan Costello, Elena Redondo Castro, Ilgiz A Mufazalov, Athina Papaemmanouil, Nancy J Rothwell, Stuart Allan, Ari Waisman, Emmanuel Pinteaux, Werner Müller

    Research output: Contribution to journalArticlepeer-review

    Abstract

    IL-1 is a key cytokine known to drive chronic inflammation and to regulate many physiological, immunological and neuroimmunological responses via actions on diverse cell types of the body. To determine the mechanisms of IL-1 actions as part of the inflammatory response in vivo, we generated a conditional IL-1 receptor 1 (IL-1R1) mouse mutant using the Cre/LoxP system (IL-1R1(fl/fl) ). In the mutant generated, exon 5, which encodes part of the extracellular binding region of the receptor, is flanked by LoxP sites, thereby inactivating the two previously described functional IL-1R1 gene transcripts after Cre-mediated recombination. Using keratin 14-Cre driver mice, new IL-1R1 deficient (-/-) mice were subsequently generated, in which all signaling IL-1 receptor isoforms are deleted ubiquitously. Furthermore, using vav-iCre driver mice, we deleted IL-1 receptor isoforms in the haematopoietic system. In these mice, we show that both the IL-17 and IL-22 cytokine response is reduced, when mice are challenged by the helminth Trichuris muris. We are currently crossing IL-1R1(fl/fl) mice with different Cre-expressing mice in order to study mechanisms of acute and chronic inflammatory diseases. This article is protected by copyright. All rights reserved.
    Original languageEnglish
    Pages (from-to)912-918
    Number of pages7
    JournalEuropean journal of immunology
    Volume46
    Issue number4
    Early online date22 Dec 2015
    DOIs
    Publication statusPublished - Apr 2016

    Keywords

    • Cre/loxP
    • IL-17
    • Immune regulation
    • Infection
    • Trichuris muris

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