Characterization of bovine seminal plasma by proteomics

Van C. Kelly; Sulee Kuy; David J. Palmer; Zhenzhong Xu; Stephen R. Davis; Garth Cooper

doi:10.1002/pmic.200500830

Characterization of bovine seminal plasma by proteomics

Van C. Kelly, Sulee Kuy, David J. Palmer, Zhenzhong Xu, Stephen R. Davis, Garth Cooper

Division of Cardiovascular Sciences (L5)

Research output: Contribution to journal › Article › peer-review

Abstract

Previous investigations of bovine seminal plasma (BSP) have revealed the identities of the three major proteins, BSP-PDC109, BSP-A3 and BSP-30 kDa, which together constitute about half of the total protein, as well as about 30 of the minor proteins. Analyses of BSP by 2-DE have revealed about 250 protein spots, suggesting that much of the BSP proteome remains undescribed. In this study, BSP has been analyzed by 2-D LC-based and SDS-PAGE-based proteomic methods. Ninety-nine proteins were identified, including 49 minor proteins that have not previously been described in seminal plasma of any species. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

Original language	English
Pages (from-to)	5826-5833
Number of pages	7
Journal	Proteomics
Volume	6
Issue number	21
DOIs	https://doi.org/10.1002/pmic.200500830
Publication status	Published - Nov 2006

Keywords

Bovine
Proteome analysis
Seminal plasma

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10.1002/pmic.200500830

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title = "Characterization of bovine seminal plasma by proteomics",

abstract = "Previous investigations of bovine seminal plasma (BSP) have revealed the identities of the three major proteins, BSP-PDC109, BSP-A3 and BSP-30 kDa, which together constitute about half of the total protein, as well as about 30 of the minor proteins. Analyses of BSP by 2-DE have revealed about 250 protein spots, suggesting that much of the BSP proteome remains undescribed. In this study, BSP has been analyzed by 2-D LC-based and SDS-PAGE-based proteomic methods. Ninety-nine proteins were identified, including 49 minor proteins that have not previously been described in seminal plasma of any species. {\textcopyright} 2006 Wiley-VCH Verlag GmbH & Co. KGaA.",

keywords = "Bovine, Proteome analysis, Seminal plasma",

author = "Kelly, {Van C.} and Sulee Kuy and Palmer, {David J.} and Zhenzhong Xu and Davis, {Stephen R.} and Garth Cooper",

year = "2006",

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doi = "10.1002/pmic.200500830",

language = "English",

volume = "6",

pages = "5826--5833",

journal = "Proteomics",

issn = "1615-9861",

publisher = "John Wiley & Sons Ltd",

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TY - JOUR

T1 - Characterization of bovine seminal plasma by proteomics

AU - Kelly, Van C.

AU - Kuy, Sulee

AU - Palmer, David J.

AU - Xu, Zhenzhong

AU - Davis, Stephen R.

AU - Cooper, Garth

PY - 2006/11

Y1 - 2006/11

N2 - Previous investigations of bovine seminal plasma (BSP) have revealed the identities of the three major proteins, BSP-PDC109, BSP-A3 and BSP-30 kDa, which together constitute about half of the total protein, as well as about 30 of the minor proteins. Analyses of BSP by 2-DE have revealed about 250 protein spots, suggesting that much of the BSP proteome remains undescribed. In this study, BSP has been analyzed by 2-D LC-based and SDS-PAGE-based proteomic methods. Ninety-nine proteins were identified, including 49 minor proteins that have not previously been described in seminal plasma of any species. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

AB - Previous investigations of bovine seminal plasma (BSP) have revealed the identities of the three major proteins, BSP-PDC109, BSP-A3 and BSP-30 kDa, which together constitute about half of the total protein, as well as about 30 of the minor proteins. Analyses of BSP by 2-DE have revealed about 250 protein spots, suggesting that much of the BSP proteome remains undescribed. In this study, BSP has been analyzed by 2-D LC-based and SDS-PAGE-based proteomic methods. Ninety-nine proteins were identified, including 49 minor proteins that have not previously been described in seminal plasma of any species. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

KW - Bovine

KW - Proteome analysis

KW - Seminal plasma

U2 - 10.1002/pmic.200500830

DO - 10.1002/pmic.200500830

M3 - Article

C2 - 17001600

SN - 1615-9861

VL - 6

SP - 5826

EP - 5833

JO - Proteomics

JF - Proteomics

IS - 21

ER -

Characterization of bovine seminal plasma by proteomics

Abstract

Keywords

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