Characterization of dentine structure in three dimensions using FIB-SEM

J. S. Earl; R. K. Leary; J. S. Perrin; R. Brydson; J. P. Harrington; K. Markowitz; S. J. Milne

doi:10.1111/j.1365-2818.2010.03396.x

Characterization of dentine structure in three dimensions using FIB-SEM

J. S. Earl, R. K. Leary, J. S. Perrin, R. Brydson, J. P. Harrington, K. Markowitz, S. J. Milne

Research output: Contribution to journal › Article › peer-review

Abstract

All biological tissues are three dimensional and contain structures that span a range of length scales from nanometres through to hundreds of millimetres. These are not ideally suited to current three-dimensional characterization techniques such as X-ray or transmission electron tomography. Such detailed morphological analysis is critical to understanding the structural features relevant to tissue function and designing therapeutic strategies intended to address structural deficiencies encountered in pathological states. We show that use of focused ion beam milling combined with scanning electron microscopy can provide three-dimensional information at nanometre resolution from biologically relevant volumes of material, in this case dentine. © 2010 The Royal Microscopical Society.

Original language	English
Pages (from-to)	1-5
Number of pages	4
Journal	Journal of Microscopy
Volume	240
Issue number	1
DOIs	https://doi.org/10.1111/j.1365-2818.2010.03396.x
Publication status	Published - Oct 2010

Keywords

Dentine
focused ion beam
scanning electron microscopy
slice and view
three dimensional

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10.1111/j.1365-2818.2010.03396.x

http://dx.doi.org/10.1111/j.1365-2818.2010.03396.x

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title = "Characterization of dentine structure in three dimensions using FIB-SEM",

abstract = "All biological tissues are three dimensional and contain structures that span a range of length scales from nanometres through to hundreds of millimetres. These are not ideally suited to current three-dimensional characterization techniques such as X-ray or transmission electron tomography. Such detailed morphological analysis is critical to understanding the structural features relevant to tissue function and designing therapeutic strategies intended to address structural deficiencies encountered in pathological states. We show that use of focused ion beam milling combined with scanning electron microscopy can provide three-dimensional information at nanometre resolution from biologically relevant volumes of material, in this case dentine. {\textcopyright} 2010 The Royal Microscopical Society.",

keywords = "Dentine, focused ion beam, scanning electron microscopy, slice and view, three dimensional",

author = "Earl, {J. S.} and Leary, {R. K.} and Perrin, {J. S.} and R. Brydson and Harrington, {J. P.} and K. Markowitz and Milne, {S. J.}",

year = "2010",

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language = "English",

volume = "240",

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T1 - Characterization of dentine structure in three dimensions using FIB-SEM

AU - Earl, J. S.

AU - Leary, R. K.

AU - Perrin, J. S.

AU - Brydson, R.

AU - Harrington, J. P.

AU - Markowitz, K.

AU - Milne, S. J.

PY - 2010/10

Y1 - 2010/10

N2 - All biological tissues are three dimensional and contain structures that span a range of length scales from nanometres through to hundreds of millimetres. These are not ideally suited to current three-dimensional characterization techniques such as X-ray or transmission electron tomography. Such detailed morphological analysis is critical to understanding the structural features relevant to tissue function and designing therapeutic strategies intended to address structural deficiencies encountered in pathological states. We show that use of focused ion beam milling combined with scanning electron microscopy can provide three-dimensional information at nanometre resolution from biologically relevant volumes of material, in this case dentine. © 2010 The Royal Microscopical Society.

AB - All biological tissues are three dimensional and contain structures that span a range of length scales from nanometres through to hundreds of millimetres. These are not ideally suited to current three-dimensional characterization techniques such as X-ray or transmission electron tomography. Such detailed morphological analysis is critical to understanding the structural features relevant to tissue function and designing therapeutic strategies intended to address structural deficiencies encountered in pathological states. We show that use of focused ion beam milling combined with scanning electron microscopy can provide three-dimensional information at nanometre resolution from biologically relevant volumes of material, in this case dentine. © 2010 The Royal Microscopical Society.

KW - Dentine

KW - focused ion beam

KW - scanning electron microscopy

KW - slice and view

KW - three dimensional

U2 - 10.1111/j.1365-2818.2010.03396.x

DO - 10.1111/j.1365-2818.2010.03396.x

M3 - Article

SN - 1365-2818

VL - 240

SP - 1

EP - 5

JO - Journal of Microscopy

JF - Journal of Microscopy

IS - 1

ER -

Characterization of dentine structure in three dimensions using FIB-SEM

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Keywords

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