Characterization of isolated ventricular myocytes from adult zebrafish (Danio rerio)

Fabien Brette, Guillermo Luxan, Caroline Cros, Hayley Dixey, Christopher Wilson, Holly A. Shiels

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The zebrafish is widely used for human related disease studies. Surprisingly, there is no information about the electrical activity of single myocytes freshly isolated from adult zebrafish ventricle. In this study, we present an enzymatic method to isolate ventricular myocytes from zebrafish heart that yield a large number of calcium tolerant cells. Ventricular myocytes from zebrafish were imaged using light and confocal microscopy. Myocytes were mostly rod shaped and responded by vigorous contraction to field electrical stimulation. Whole cell configuration of the patch clamp technique was used to record electrophysiological characteristics of myocytes. Action potentials present a long duration and a plateau phase and action potential duration decreases when increasing stimulation frequency (as observed in larger mammals). Together these results indicate that zebrafish is a species ideally suited for investigation of ion channels related mutation screening of cardiac alteration important in human. © 2008 Elsevier Inc. All rights reserved.
    Original languageEnglish
    Pages (from-to)143-146
    Number of pages3
    JournalBiochemical and Biophysical Research Communications
    Volume374
    Issue number1
    DOIs
    Publication statusPublished - 12 Sept 2008

    Keywords

    • Cardiac myocytes
    • Enzymatic isolation
    • Heart
    • Ion channels
    • Zebrafish

    Fingerprint

    Dive into the research topics of 'Characterization of isolated ventricular myocytes from adult zebrafish (Danio rerio)'. Together they form a unique fingerprint.

    Cite this