Characterization of Short Tandem Repeats from Thirty-One Human Telomeres

Marjorie Rosenberg; Lester Hui; Junli Ma; Harris C. Nusbaum; Kevin Clark; Louise Robinson; Laura Dziadzio; Pamela M. Swain; Tim Keith; Thomas J. Hudson; Leslie G Biesecker; Jonathan Flint

doi:10.1101/gr.7.9.917

Characterization of Short Tandem Repeats from Thirty-One Human Telomeres

Marjorie Rosenberg, Lester Hui, Junli Ma, Harris C. Nusbaum, Kevin Clark, Louise Robinson, Laura Dziadzio, Pamela M. Swain, Tim Keith, Thomas J. Hudson, Leslie G Biesecker, Jonathan Flint

Division of Psychology & Mental Health (L5)

National Human Genome Research Institute

Research output: Contribution to journal › Article › peer-review

Abstract

Completion of genetic and physical maps requires markers from the ends (telomeres) of every human chromosome. We have searched for short tandem repeats (microsatellites) in cosmid and P1 clones and generated 661 sequence-tagged sites (STS) from the terminal 300 kb of 31 human chromosome ends. PCR assays were successfully designed for 58 microsatellites and mapped both genetically and on radiation hybrids (RHs) to confirm their telomeric location. Sequence analysis revealed marked variation in sequence composition, consistent with the hypothesis that even very highly GC-rich chromosome bands (the T bands) are not homogenous. The STSs that we have generated will be a necessary resource for the construction of physical maps of these complex regions of the genome.

[Information about the microsatellites is available electronically at http://www.cshl.org/gr and sequence has been deposited in the Genome Database (GDB).]

Original language	English
Pages (from-to)	917-923
Number of pages	7
Journal	Genome research
Volume	7
DOIs	https://doi.org/10.1101/gr.7.9.917
Publication status	Published - 1 Sept 1997

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title = "Characterization of Short Tandem Repeats from Thirty-One Human Telomeres",

abstract = "Completion of genetic and physical maps requires markers from the ends (telomeres) of every human chromosome. We have searched for short tandem repeats (microsatellites) in cosmid and P1 clones and generated 661 sequence-tagged sites (STS) from the terminal 300 kb of 31 human chromosome ends. PCR assays were successfully designed for 58 microsatellites and mapped both genetically and on radiation hybrids (RHs) to confirm their telomeric location. Sequence analysis revealed marked variation in sequence composition, consistent with the hypothesis that even very highly GC-rich chromosome bands (the T bands) are not homogenous. The STSs that we have generated will be a necessary resource for the construction of physical maps of these complex regions of the genome.[Information about the microsatellites is available electronically at http://www.cshl.org/gr and sequence has been deposited in the Genome Database (GDB).]",

author = "Marjorie Rosenberg and Lester Hui and Junli Ma and Nusbaum, {Harris C.} and Kevin Clark and Louise Robinson and Laura Dziadzio and Swain, {Pamela M.} and Tim Keith and Hudson, {Thomas J.} and Biesecker, {Leslie G} and Jonathan Flint",

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doi = "10.1101/gr.7.9.917",

language = "English",

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TY - JOUR

T1 - Characterization of Short Tandem Repeats from Thirty-One Human Telomeres

AU - Rosenberg, Marjorie

AU - Hui, Lester

AU - Ma, Junli

AU - Nusbaum, Harris C.

AU - Clark, Kevin

AU - Robinson, Louise

AU - Dziadzio, Laura

AU - Swain, Pamela M.

AU - Keith, Tim

AU - Hudson, Thomas J.

AU - Biesecker, Leslie G

AU - Flint, Jonathan

PY - 1997/9/1

Y1 - 1997/9/1

N2 - Completion of genetic and physical maps requires markers from the ends (telomeres) of every human chromosome. We have searched for short tandem repeats (microsatellites) in cosmid and P1 clones and generated 661 sequence-tagged sites (STS) from the terminal 300 kb of 31 human chromosome ends. PCR assays were successfully designed for 58 microsatellites and mapped both genetically and on radiation hybrids (RHs) to confirm their telomeric location. Sequence analysis revealed marked variation in sequence composition, consistent with the hypothesis that even very highly GC-rich chromosome bands (the T bands) are not homogenous. The STSs that we have generated will be a necessary resource for the construction of physical maps of these complex regions of the genome.[Information about the microsatellites is available electronically at http://www.cshl.org/gr and sequence has been deposited in the Genome Database (GDB).]

AB - Completion of genetic and physical maps requires markers from the ends (telomeres) of every human chromosome. We have searched for short tandem repeats (microsatellites) in cosmid and P1 clones and generated 661 sequence-tagged sites (STS) from the terminal 300 kb of 31 human chromosome ends. PCR assays were successfully designed for 58 microsatellites and mapped both genetically and on radiation hybrids (RHs) to confirm their telomeric location. Sequence analysis revealed marked variation in sequence composition, consistent with the hypothesis that even very highly GC-rich chromosome bands (the T bands) are not homogenous. The STSs that we have generated will be a necessary resource for the construction of physical maps of these complex regions of the genome.[Information about the microsatellites is available electronically at http://www.cshl.org/gr and sequence has been deposited in the Genome Database (GDB).]

UR - http://dx.doi.org/10.1101/gr.7.9.917

U2 - 10.1101/gr.7.9.917

DO - 10.1101/gr.7.9.917

M3 - Article

SN - 1549-5469

VL - 7

SP - 917

EP - 923

JO - Genome research

JF - Genome research

ER -

Characterization of Short Tandem Repeats from Thirty-One Human Telomeres

Abstract

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