Differential scanning calorimetry (DSC), Fourier-transform infrared FTIR) spectroscopy and transmission electron microscopy (TEM) have been used to determine the influence of β-cyclodextrin (β-CyD), hydroxypropyl-β-CyD (HP-β-CyD) and γ-CyD on the structural properties of the stratum corneum from the hairless mouse. Some modest changes in the stratum corneum lipid transition temperature were induced by HP-β-CyD and blue shifts were observed in the FTIR spectra of the C-H asymmetric and symmetric stretching of the lipids from the stratum corneum. Results from TEM studies indicated that HP-β-CyD caused removal and possible disorganization of the lipid matrix that envelopes the corneocytes of the stratum corneum, whereas no effect was seen after treatment of the samples with β-CyD and γ-CyD. These results suggest that HP-β-CyD can increase the permeability of the stratum corneum possibly as a result of extraction of lipids, and might thus enhance drug permeation through the skin.
|Number of pages
|Journal of Pharmacy and Pharmacology
|Published - Apr 1997