Characterization of the three Arabidopsis thaliana RAD21 cohesins reveals differential responses to ionizing radiation

J. A. Da Costa-Nunes; A. M. Bhatt; S. O'Shea; C. E. West; C. M. Bray; U. Grossniklaus; H. G. Dickinson

doi:10.1093/jxb/erj083

Characterization of the three Arabidopsis thaliana RAD21 cohesins reveals differential responses to ionizing radiation

J. A. Da Costa-Nunes, A. M. Bhatt, S. O'Shea, C. E. West, C. M. Bray, U. Grossniklaus, H. G. Dickinson

Research output: Contribution to journal › Article › peer-review

Abstract

The RAD21/REC8 gene family has been implicated in sister chromatid cohesion and DNA repair in several organisms. Unlike most eukaryotes, Arabidopsis thaliana has three RAD21 gene homologues, and their cloning and characterization are reported here. All three genes, AtRAD21.1, AtRAD21.2, and AtRAD21.3, are expressed in tissues rich in cells undergoing cell division, and AtRAD21.3 shows the highest relative level of expression. An increase in steady-state levels of AtRAD21.1 transcript was also observed, specifically after the induction of DNA damage. Phenotypic analysis of the atrad21.1 and atrad21.3 mutants revealed that neither of the single mutants was lethal, probably due to the redundancy in function of the AtRAD21 genes. However, AtRAD21.1 plays a critical role in recovery from DNA damage during seed imbibition, prior to germination, as atrad21.1 mutant seeds are hypersensitive to radiation damage. © The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.

Original language	English
Pages (from-to)	971-983
Number of pages	12
Journal	Journal of Experimental Botany
Volume	57
Issue number	4
DOIs	https://doi.org/10.1093/jxb/erj083
Publication status	Published - Mar 2006

Keywords

Arabidopsis
AtRAD21
Cohesins
DNA-damage
Double-strand-break
Ionizing radiation
Seed

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10.1093/jxb/erj083

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@article{0c08391017fc4aabb512e54c5af236ae,

title = "Characterization of the three Arabidopsis thaliana RAD21 cohesins reveals differential responses to ionizing radiation",

abstract = "The RAD21/REC8 gene family has been implicated in sister chromatid cohesion and DNA repair in several organisms. Unlike most eukaryotes, Arabidopsis thaliana has three RAD21 gene homologues, and their cloning and characterization are reported here. All three genes, AtRAD21.1, AtRAD21.2, and AtRAD21.3, are expressed in tissues rich in cells undergoing cell division, and AtRAD21.3 shows the highest relative level of expression. An increase in steady-state levels of AtRAD21.1 transcript was also observed, specifically after the induction of DNA damage. Phenotypic analysis of the atrad21.1 and atrad21.3 mutants revealed that neither of the single mutants was lethal, probably due to the redundancy in function of the AtRAD21 genes. However, AtRAD21.1 plays a critical role in recovery from DNA damage during seed imbibition, prior to germination, as atrad21.1 mutant seeds are hypersensitive to radiation damage. {\textcopyright} The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.",

keywords = "Arabidopsis, AtRAD21, Cohesins, DNA-damage, Double-strand-break, Ionizing radiation, Seed",

author = "{Da Costa-Nunes}, {J. A.} and Bhatt, {A. M.} and S. O'Shea and West, {C. E.} and Bray, {C. M.} and U. Grossniklaus and Dickinson, {H. G.}",

year = "2006",

month = mar,

doi = "10.1093/jxb/erj083",

language = "English",

volume = "57",

pages = "971--983",

journal = "Journal of Experimental Botany",

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publisher = "Oxford University Press",

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T1 - Characterization of the three Arabidopsis thaliana RAD21 cohesins reveals differential responses to ionizing radiation

AU - Da Costa-Nunes, J. A.

AU - Bhatt, A. M.

AU - O'Shea, S.

AU - West, C. E.

AU - Bray, C. M.

AU - Grossniklaus, U.

AU - Dickinson, H. G.

PY - 2006/3

Y1 - 2006/3

N2 - The RAD21/REC8 gene family has been implicated in sister chromatid cohesion and DNA repair in several organisms. Unlike most eukaryotes, Arabidopsis thaliana has three RAD21 gene homologues, and their cloning and characterization are reported here. All three genes, AtRAD21.1, AtRAD21.2, and AtRAD21.3, are expressed in tissues rich in cells undergoing cell division, and AtRAD21.3 shows the highest relative level of expression. An increase in steady-state levels of AtRAD21.1 transcript was also observed, specifically after the induction of DNA damage. Phenotypic analysis of the atrad21.1 and atrad21.3 mutants revealed that neither of the single mutants was lethal, probably due to the redundancy in function of the AtRAD21 genes. However, AtRAD21.1 plays a critical role in recovery from DNA damage during seed imbibition, prior to germination, as atrad21.1 mutant seeds are hypersensitive to radiation damage. © The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.

AB - The RAD21/REC8 gene family has been implicated in sister chromatid cohesion and DNA repair in several organisms. Unlike most eukaryotes, Arabidopsis thaliana has three RAD21 gene homologues, and their cloning and characterization are reported here. All three genes, AtRAD21.1, AtRAD21.2, and AtRAD21.3, are expressed in tissues rich in cells undergoing cell division, and AtRAD21.3 shows the highest relative level of expression. An increase in steady-state levels of AtRAD21.1 transcript was also observed, specifically after the induction of DNA damage. Phenotypic analysis of the atrad21.1 and atrad21.3 mutants revealed that neither of the single mutants was lethal, probably due to the redundancy in function of the AtRAD21 genes. However, AtRAD21.1 plays a critical role in recovery from DNA damage during seed imbibition, prior to germination, as atrad21.1 mutant seeds are hypersensitive to radiation damage. © The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.

KW - Arabidopsis

KW - AtRAD21

KW - Cohesins

KW - DNA-damage

KW - Double-strand-break

KW - Ionizing radiation

KW - Seed

U2 - 10.1093/jxb/erj083

DO - 10.1093/jxb/erj083

M3 - Article

C2 - 16488915

SN - 0022-0957

VL - 57

SP - 971

EP - 983

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

IS - 4

ER -

Characterization of the three Arabidopsis thaliana RAD21 cohesins reveals differential responses to ionizing radiation

Abstract

Keywords

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