Characterization of vitellogenin in the shrimp Metapenaeus ensis: Expression studies and hormonal regulation of MeVg1 transcription in vitro

Shirley H K Tiu, Jerome H L Hui, Jian Guo He, Stephen S. Tobe, Siu Ming Chan

    Research output: Contribution to journalArticlepeer-review

    Abstract

    During gonad maturation, female shrimp accumulate the major egg yolk protein vitellin (Vn) in premolt stage, and the process of molting and reproduction is synchronized. Using a polyclonal anti-Vn antibody, immunopositive signals could be detected in the ovary and among the proteins secreted by the hepatopancreas by Western blot. In the ovary, Vn immunoreactivity was located in the posterior lobe. Hepatopancreas proteins with sizes identical to ovary vitellogenin (Vg) subunits (i.e., 78 and 157 kDa) were immunoreactive to the Vn antibody and these proteins included amino acid sequences identical to parts of the MeVg1 precursor. A major 7.8 kb MeVg1 transcript, was detected in the ovary. In the hepatopancreas, the transcripts were primarily small (
    Original languageEnglish
    Pages (from-to)424-436
    Number of pages12
    JournalMolecular Reproduction and Development
    Volume73
    Issue number4
    DOIs
    Publication statusPublished - Apr 2006

    Keywords

    • Alternative gene expression
    • Hepatopancreas
    • Ovary
    • Shrimp
    • Vitellin
    • Vitellogenin

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