Abstract
During gonad maturation, female shrimp accumulate the major egg yolk protein vitellin (Vn) in premolt stage, and the process of molting and reproduction is synchronized. Using a polyclonal anti-Vn antibody, immunopositive signals could be detected in the ovary and among the proteins secreted by the hepatopancreas by Western blot. In the ovary, Vn immunoreactivity was located in the posterior lobe. Hepatopancreas proteins with sizes identical to ovary vitellogenin (Vg) subunits (i.e., 78 and 157 kDa) were immunoreactive to the Vn antibody and these proteins included amino acid sequences identical to parts of the MeVg1 precursor. A major 7.8 kb MeVg1 transcript, was detected in the ovary. In the hepatopancreas, the transcripts were primarily small (
Original language | English |
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Pages (from-to) | 424-436 |
Number of pages | 12 |
Journal | Molecular Reproduction and Development |
Volume | 73 |
Issue number | 4 |
DOIs | |
Publication status | Published - Apr 2006 |
Keywords
- Alternative gene expression
- Hepatopancreas
- Ovary
- Shrimp
- Vitellin
- Vitellogenin