Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope

Aélig Robin; Valentin Köhler; Alison Jones; Afruja Ali; Paul P. Kelly; Elaine O'Reilly; Nicholas J. Turner; Sabine L. Flitsch

doi:10.3762/bjoc.7.173

Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope

Aélig Robin, Valentin Köhler, Alison Jones, Afruja Ali, Paul P. Kelly, Elaine O'Reilly, Nicholas J. Turner, Sabine L. Flitsch

Research output: Contribution to journal › Article › peer-review

Abstract

A high-throughput screening protocol for evaluating chimeric, self-sufficient P450 biocatalysts and their mutants against a panel of substrates was developed, leading to the identification of a number of novel biooxidation activities. © 2011 Robin et al.

Original language	English
Article number	7
Pages (from-to)	1494-1498
Number of pages	4
Journal	Beilstein Journal of Organic Chemistry
Volume	7
DOIs	https://doi.org/10.3762/bjoc.7.173
Publication status	Published - 2 Nov 2011

Keywords

Biocatalysis
C-H activation
High-throughput screening
P450 monooxygenase
Substrate engineering

Access to Document

10.3762/bjoc.7.173

http://www.beilstein-journals.org/bjoc/content/pdf/1860-5397-7-173.pdf

Cite this

@article{681965df6e4f4836ab2e02b35f3ae536,

title = "Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope",

abstract = "A high-throughput screening protocol for evaluating chimeric, self-sufficient P450 biocatalysts and their mutants against a panel of substrates was developed, leading to the identification of a number of novel biooxidation activities. {\textcopyright} 2011 Robin et al.",

keywords = "Biocatalysis, C-H activation, High-throughput screening, P450 monooxygenase, Substrate engineering",

author = "A{\'e}lig Robin and Valentin K{\"o}hler and Alison Jones and Afruja Ali and Kelly, {Paul P.} and Elaine O'Reilly and Turner, {Nicholas J.} and Flitsch, {Sabine L.}",

year = "2011",

month = nov,

day = "2",

doi = "10.3762/bjoc.7.173",

language = "English",

volume = "7",

pages = "1494--1498",

journal = "Beilstein Journal of Organic Chemistry",

issn = "1860-5397",

publisher = "Beilstein-Institut ",

}

TY - JOUR

T1 - Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope

AU - Robin, Aélig

AU - Köhler, Valentin

AU - Jones, Alison

AU - Ali, Afruja

AU - Kelly, Paul P.

AU - O'Reilly, Elaine

AU - Turner, Nicholas J.

AU - Flitsch, Sabine L.

PY - 2011/11/2

Y1 - 2011/11/2

N2 - A high-throughput screening protocol for evaluating chimeric, self-sufficient P450 biocatalysts and their mutants against a panel of substrates was developed, leading to the identification of a number of novel biooxidation activities. © 2011 Robin et al.

AB - A high-throughput screening protocol for evaluating chimeric, self-sufficient P450 biocatalysts and their mutants against a panel of substrates was developed, leading to the identification of a number of novel biooxidation activities. © 2011 Robin et al.

KW - Biocatalysis

KW - C-H activation

KW - High-throughput screening

KW - P450 monooxygenase

KW - Substrate engineering

U2 - 10.3762/bjoc.7.173

DO - 10.3762/bjoc.7.173

M3 - Article

SN - 1860-5397

VL - 7

SP - 1494

EP - 1498

JO - Beilstein Journal of Organic Chemistry

JF - Beilstein Journal of Organic Chemistry

M1 - 7

ER -

Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope

Abstract

Keywords

Access to Document

Fingerprint

Cite this