Circadian changes in PACAP type 1 (PAC1) receptor mRNA in the rat suprachiasmatic and supraoptic nuclei

Felino Ramon A Cagampang, Hugh D. Piggins, W. John Sheward, Anthony J. Harmar, Clive W. Coen

    Research output: Contribution to journalArticlepeer-review

    Abstract

    A receptor for pituitary adenylate cyclase activating polypeptide (PACAP), denoted as PAC1, is expressed in the suprachiasmatic nuclei (SCN). Since the circadian clock demonstrates phase-dependent sensitivity to PACAP, we have used in situ hybridization histochemistry to examine whether PAC1 mRNA is differentially expressed in the rat SCN across the 24-h cycle. There was a significant variation in PAC1 mRNA within the SCN and supraoptic nuclei during the light-dark cycle and in constant darkness, with peaks at the middle of both the real and subjective day and night; no significant variation was observed in the cingulate cortex. The results suggest that the phase-dependent actions of PACAP on the clock may involve phase-specific changes in the availability of PAC1 receptors within the SCN.
    Original languageEnglish
    Pages (from-to)218-222
    Number of pages4
    JournalBrain research
    Volume813
    Issue number1
    DOIs
    Publication statusPublished - 30 Nov 1998

    Keywords

    • Circadian rhythm
    • In situ hybridization histochemistry
    • PAC1
    • PACAP
    • Pituitary adenylate cyclase-activating polypeptide receptor
    • Suprachiasmatic nuclei
    • Supraoptic nuclei

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