Classification and cluster analysis of complex time-of-flight secondary ion mass spectrometry for biological samples

Xue Tian, Stephen E. Reichenbach, Qingping Tao, Alex Henderson

    Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

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    Identifying and separating subtly different biological samples is one of the most critical tasks in biological analysis. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is becoming a popular and important technique in the analysis of biological samples, because it can detect molecular information and characterize chemical composition. ToF-SIMS spectra of biological samples are enormously complex with large mass ranges and many peaks. As a result the classification and cluster analysis are challenging. This study presents a new classification algorithm, the most similar neighbor with a probability-based spectrum similarity measure (MSNPSSM), which uses all the information in the entire ToFSIMS spectra. MSN-PSSM is applied to automatically classify bacterial samples which are major causal agents of urinary tract infections. Experimental results show that MSN-PSSM is an accurate classification algorithm. It outperforms traditional techniques such as decision trees, principal component analysis (PCA) with discriminant function analysis (DFA), and soft independent modeling of class analogy (SIMCA). This study also applies a modern clustering algorithm, normalized spectral clustering, to automatically cluster the bacterial samples at the species level. Experimental results demonstrate that normalized spectral clustering is able to show accurate quantitative separations. It outperforms traditional techniques such as hierarchical clustering analysis, kmeans, and PCA with k-means. Copyright© (2009) by the International Society for Research in Science and Technology.
    Original languageEnglish
    Title of host publicationInternational Conference on Bioinformatics, Computational Biology, Genomics and Chemoinformatics 2009, BCBGC 2009
    Number of pages7
    Publication statusPublished - 2009

    Research Beacons, Institutes and Platforms

    • Manchester Institute of Biotechnology


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