Clinical features of photodamaged human skin are associated with a reduction in collagen VII

N.M. Craven, R.E.B Watson, Carolyn Jones, C.A. Shuttleworth, Cay Kielty, CEM Griffiths

Research output: Contribution to journalArticlepeer-review

Abstract

Chronically sun-exposed or photodamaged human skin is characterized by a number of clinical features, including wrinkles. However, little is known about the molecular mechanisms that underlie these features. We investigated the hypothesis that the mechanism of wrinkle formation may involve loss of anchoring fibrils, composed mainly of collagen VII, which are important in maintaining dermal-epidermal junction integrity. Ten volunteers with moderate to severe photodamage of dorsal forearm skin were recruited to the study. Using immunohistochemistry, transmission electron microscopy and in situ hybridization, we compared collagen VII protein and mRNA content of photodamaged forearm skin with that of sun-protected hip and upper inner arm skin from the same subjects. Numbers of anchoring fibrils per linear μm of basement membrane (mean ± SEM) were significantly lower in photodamaged skin (1.79 ± 0.10) as compared with sun-protected hip (2.28 ± 0.11) and upper inner arm skin (2.21 ± 0.10) (P
Original languageUndefined
Pages (from-to)344-350
Number of pages7
JournalBritish Journal of Dermatology
Volume137
Issue number3
Publication statusPublished - 1997

Keywords

  • collagen type 7
  • messenger rna, adult
  • aged
  • article
  • clinical article
  • clinical feature
  • controlled study
  • female
  • human
  • human tissue
  • immunohistochemistry
  • in situ hybridization
  • male
  • photodermatosis
  • priority journal
  • skin biopsy
  • sun exposure
  • transmission electron microscopy, Aged
  • Aged, 80 and over
  • Collagen
  • Female
  • Gene Expression
  • Humans
  • Immunoenzyme Techniques
  • In Situ Hybridization
  • Male
  • Microscopy, Electron
  • Middle Aged
  • Radiation Injuries
  • RNA, Messenger
  • Skin
  • Skin Aging
  • Sunlight

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