Cloning and characterization of a PI-like MADS-box gene in Phalaenopsis orchid

Bin Guo, Saiyin Hexige, Tian Zhang, Jon K. Pittman, Donghong Chen, Feng Ming

    Research output: Contribution to journalArticlepeer-review


    The highly evolved flowers of orchids have colorful sepals and fused columns that offer an opportunity to discover new genes involved in floral development in monocotyledon species. In this investigation, we cloned and characterized the homologous PISTALLATA-like (PI-like) gene PhPI15 (Phalaenopsis PI STILLATA # 15), from the Phalaenopsis hybrid cultivar. The protein sequence encoded by PhPI15 contains a typical PI-motif. Its sequence also formed a subclade with other monocot PI-type genes in phylogenetic analysis. Southern analysis showed that PhPI15 was present in the Phalaenopsis orchid genome as a single copy. Furthermore, it was expressed in all the whorls of the Phalaenopsis flower, while no expression was detected in vegetative organs. The flowers of transgenic tobacco plants ectopically expressing PhPI15 showed male-sterile phenotypes. Thus, as a Class-B MADS-box gene, PhPI15 specifies floral organ identity in orchids.
    Original languageEnglish
    Pages (from-to)845-852
    Number of pages7
    JournalJournal of Biochemistry and Molecular Biology
    Issue number6
    Publication statusPublished - Nov 2007


    • In situ hybridization
    • MADS-box gene
    • Phalaenopsis orchid
    • PI


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