Cloning and characterization of a PI-like MADS-box gene in Phalaenopsis orchid

Bin Guo; Saiyin Hexige; Tian Zhang; Jon K. Pittman; Donghong Chen; Feng Ming

doi:10.5483/BMBRep.2007.40.6.845

Cloning and characterization of a PI-like MADS-box gene in Phalaenopsis orchid

Bin Guo, Saiyin Hexige, Tian Zhang, Jon K. Pittman, Donghong Chen, Feng Ming

Research output: Contribution to journal › Article › peer-review

Abstract

The highly evolved flowers of orchids have colorful sepals and fused columns that offer an opportunity to discover new genes involved in floral development in monocotyledon species. In this investigation, we cloned and characterized the homologous PISTALLATA-like (PI-like) gene PhPI15 (Phalaenopsis PI STILLATA # 15), from the Phalaenopsis hybrid cultivar. The protein sequence encoded by PhPI15 contains a typical PI-motif. Its sequence also formed a subclade with other monocot PI-type genes in phylogenetic analysis. Southern analysis showed that PhPI15 was present in the Phalaenopsis orchid genome as a single copy. Furthermore, it was expressed in all the whorls of the Phalaenopsis flower, while no expression was detected in vegetative organs. The flowers of transgenic tobacco plants ectopically expressing PhPI15 showed male-sterile phenotypes. Thus, as a Class-B MADS-box gene, PhPI15 specifies floral organ identity in orchids.

Original language	English
Pages (from-to)	845-852
Number of pages	7
Journal	Journal of Biochemistry and Molecular Biology
Volume	40
Issue number	6
DOIs	https://doi.org/10.5483/BMBRep.2007.40.6.845
Publication status	Published - Nov 2007

Keywords

In situ hybridization
MADS-box gene
Phalaenopsis orchid
PI

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title = "Cloning and characterization of a PI-like MADS-box gene in Phalaenopsis orchid",

abstract = "The highly evolved flowers of orchids have colorful sepals and fused columns that offer an opportunity to discover new genes involved in floral development in monocotyledon species. In this investigation, we cloned and characterized the homologous PISTALLATA-like (PI-like) gene PhPI15 (Phalaenopsis PI STILLATA # 15), from the Phalaenopsis hybrid cultivar. The protein sequence encoded by PhPI15 contains a typical PI-motif. Its sequence also formed a subclade with other monocot PI-type genes in phylogenetic analysis. Southern analysis showed that PhPI15 was present in the Phalaenopsis orchid genome as a single copy. Furthermore, it was expressed in all the whorls of the Phalaenopsis flower, while no expression was detected in vegetative organs. The flowers of transgenic tobacco plants ectopically expressing PhPI15 showed male-sterile phenotypes. Thus, as a Class-B MADS-box gene, PhPI15 specifies floral organ identity in orchids.",

keywords = "In situ hybridization, MADS-box gene, Phalaenopsis orchid, PI",

author = "Bin Guo and Saiyin Hexige and Tian Zhang and Pittman, {Jon K.} and Donghong Chen and Feng Ming",

year = "2007",

month = nov,

doi = "10.5483/BMBRep.2007.40.6.845",

language = "English",

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journal = "Journal of Biochemistry and Molecular Biology",

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T1 - Cloning and characterization of a PI-like MADS-box gene in Phalaenopsis orchid

AU - Guo, Bin

AU - Hexige, Saiyin

AU - Zhang, Tian

AU - Pittman, Jon K.

AU - Chen, Donghong

AU - Ming, Feng

PY - 2007/11

Y1 - 2007/11

N2 - The highly evolved flowers of orchids have colorful sepals and fused columns that offer an opportunity to discover new genes involved in floral development in monocotyledon species. In this investigation, we cloned and characterized the homologous PISTALLATA-like (PI-like) gene PhPI15 (Phalaenopsis PI STILLATA # 15), from the Phalaenopsis hybrid cultivar. The protein sequence encoded by PhPI15 contains a typical PI-motif. Its sequence also formed a subclade with other monocot PI-type genes in phylogenetic analysis. Southern analysis showed that PhPI15 was present in the Phalaenopsis orchid genome as a single copy. Furthermore, it was expressed in all the whorls of the Phalaenopsis flower, while no expression was detected in vegetative organs. The flowers of transgenic tobacco plants ectopically expressing PhPI15 showed male-sterile phenotypes. Thus, as a Class-B MADS-box gene, PhPI15 specifies floral organ identity in orchids.

AB - The highly evolved flowers of orchids have colorful sepals and fused columns that offer an opportunity to discover new genes involved in floral development in monocotyledon species. In this investigation, we cloned and characterized the homologous PISTALLATA-like (PI-like) gene PhPI15 (Phalaenopsis PI STILLATA # 15), from the Phalaenopsis hybrid cultivar. The protein sequence encoded by PhPI15 contains a typical PI-motif. Its sequence also formed a subclade with other monocot PI-type genes in phylogenetic analysis. Southern analysis showed that PhPI15 was present in the Phalaenopsis orchid genome as a single copy. Furthermore, it was expressed in all the whorls of the Phalaenopsis flower, while no expression was detected in vegetative organs. The flowers of transgenic tobacco plants ectopically expressing PhPI15 showed male-sterile phenotypes. Thus, as a Class-B MADS-box gene, PhPI15 specifies floral organ identity in orchids.

KW - In situ hybridization

KW - MADS-box gene

KW - Phalaenopsis orchid

KW - PI

U2 - 10.5483/BMBRep.2007.40.6.845

DO - 10.5483/BMBRep.2007.40.6.845

M3 - Article

C2 - 18047777

SN - 0219-1024

VL - 40

SP - 845

EP - 852

JO - Journal of Biochemistry and Molecular Biology

JF - Journal of Biochemistry and Molecular Biology

IS - 6

ER -

Cloning and characterization of a PI-like MADS-box gene in Phalaenopsis orchid

Abstract

Keywords

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