TY - JOUR
T1 - Cloning and expression in Escherichia coli of the toluene dioxygenase gene from Pseudomonas putida NCIB11767
AU - Stephens, G. M.
AU - Sidebotham, J. M.
AU - Mann, N. H.
AU - Dalton, H.
N1 - CAN 110:149145 3-4 Biochemical Genetics Dep. Chem. Eng.,UMIST,Manchester,UK. Journal 0378-1097 written in English. 68651-46-7 (Indigo) Role: FORM (Formation, nonpreparative) (formation of, by Escherichia coli contg. cloned toluene dioxygenase gene from Pseudomonas putida); 9029-46-3 (Catechol 2,3-oxygenase); 9075-66-5 (Toluene dioxygenase); 63774-46-9 (Toluene cis-glycol dehydrogenase) Role: PRP (Properties) (gene for, of Pseudomonas putida, cloning and expression in Escherichia coli of); 71-43-2 (Benzene); 108-88-3 (Toluene) Role: BIOL (Biological study) (toluene dioxygenase of Pseudomonas putida induction by)
PY - 1989
Y1 - 1989
N2 - The genes encoding toluene dioxygenase, toluene cis-glycol dehydrogenase and catechol 2,3-oxygenase from Pseudomonas putida NCIB11767 were cloned and expressed in Escherichia coli HB101 on a 20 kb fragment. The recombinant strain produced indigo and a variety of other coloured products. Although the enzymes were expressed in the absence of inducers, further induction was observed in the presence of toluene or benzene, implying the presence of regulatory elements on the 20 kb insert.
AB - The genes encoding toluene dioxygenase, toluene cis-glycol dehydrogenase and catechol 2,3-oxygenase from Pseudomonas putida NCIB11767 were cloned and expressed in Escherichia coli HB101 on a 20 kb fragment. The recombinant strain produced indigo and a variety of other coloured products. Although the enzymes were expressed in the absence of inducers, further induction was observed in the presence of toluene or benzene, implying the presence of regulatory elements on the 20 kb insert.
KW - Escherichia coli (cloning and expression in, of toluene dioxygenase genes of Pseudomonas putida)
KW - Gene and Genetic element Role: BIOL (Biological study) (for toluene dioxygenase, of Pseudomonas putida, cloning and expression in Escherichia coli of)
KW - Molecular cloning (of toluene dioxygenase genes, of Pseudomonas putida, in Escherichia coli)
KW - Pseudomonas putida (toluene dioxygenase genes of, cloning and expression in Escherichia coli of)
KW - Pseudomonas toluene dioxygenase gene cloning Escherichia
KW - toluene glycol dehydrogenase Pseudomonas cloning Escherichia
KW - catechol oxygenase gene Pseudomonas cloning Escherichia
M3 - Article
SN - 1574-6968
VL - 57
SP - 295
EP - 300
JO - FEMS microbiology letters
JF - FEMS microbiology letters
IS - 3
ER -