Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor

Linda S. Park; Unja Martin; Kirsten Garka; Brian Gliniak; James P. Di Santo; Werner Muller; David A. Largaespada; Neal G. Copeland; Nancy A. Jenkins; Andrew G. Farr; Steven F. Ziegler; Philip J. Morrissey; Raymond Paxton; John E. Sims

doi:10.1084/jem.192.5.659

Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor

Linda S. Park, Unja Martin, Kirsten Garka, Brian Gliniak, James P. Di Santo, Werner Muller, David A. Largaespada, Neal G. Copeland, Nancy A. Jenkins, Andrew G. Farr, Steven F. Ziegler, Philip J. Morrissey, Raymond Paxton, John E. Sims

Research output: Contribution to journal › Article › peer-review

Abstract

The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Rα chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7Rα(-/-) mice, but did activate cells from γc(-/-) mice. Thus, the functional TSLPR requires the IL-7Rα chain, but not the γc chain for signaling.

Original language	English
Pages (from-to)	659-669
Number of pages	10
Journal	Journal of Experimental Medicine
Volume	192
Issue number	5
DOIs	https://doi.org/10.1084/jem.192.5.659
Publication status	Published - 4 Sept 2000

Keywords

Cytokine
Cytokine receptors
DNA sequence
Interleukin 2 receptor
Interleukin 7 receptor

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1084/jem.192.5.659

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Park, L. S., Martin, U., Garka, K., Gliniak, B., Di Santo, J. P., Muller, W., Largaespada, D. A., Copeland, N. G., Jenkins, N. A., Farr, A. G., Ziegler, S. F., Morrissey, P. J., Paxton, R., & Sims, J. E. (2000). Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor. Journal of Experimental Medicine, 192(5), 659-669. https://doi.org/10.1084/jem.192.5.659

@article{723a14da061848d09dabc656717fac7a,

title = "Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor",

abstract = "The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Rα chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7Rα(-/-) mice, but did activate cells from γc(-/-) mice. Thus, the functional TSLPR requires the IL-7Rα chain, but not the γc chain for signaling.",

keywords = "Cytokine, Cytokine receptors, DNA sequence, Interleukin 2 receptor, Interleukin 7 receptor",

author = "Park, {Linda S.} and Unja Martin and Kirsten Garka and Brian Gliniak and {Di Santo}, {James P.} and Werner Muller and Largaespada, {David A.} and Copeland, {Neal G.} and Jenkins, {Nancy A.} and Farr, {Andrew G.} and Ziegler, {Steven F.} and Morrissey, {Philip J.} and Raymond Paxton and Sims, {John E.}",

year = "2000",

month = sep,

day = "4",

doi = "10.1084/jem.192.5.659",

language = "English",

volume = "192",

pages = "659--669",

journal = "Journal of Experimental Medicine",

issn = "1540-9538",

publisher = "Rockefeller University Press",

number = "5",

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Park, LS, Martin, U, Garka, K, Gliniak, B, Di Santo, JP, Muller, W, Largaespada, DA, Copeland, NG, Jenkins, NA, Farr, AG, Ziegler, SF, Morrissey, PJ, Paxton, R & Sims, JE 2000, 'Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor', Journal of Experimental Medicine, vol. 192, no. 5, pp. 659-669. https://doi.org/10.1084/jem.192.5.659

TY - JOUR

T1 - Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor

AU - Park, Linda S.

AU - Martin, Unja

AU - Garka, Kirsten

AU - Gliniak, Brian

AU - Di Santo, James P.

AU - Muller, Werner

AU - Largaespada, David A.

AU - Copeland, Neal G.

AU - Jenkins, Nancy A.

AU - Farr, Andrew G.

AU - Ziegler, Steven F.

AU - Morrissey, Philip J.

AU - Paxton, Raymond

AU - Sims, John E.

PY - 2000/9/4

Y1 - 2000/9/4

N2 - The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Rα chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7Rα(-/-) mice, but did activate cells from γc(-/-) mice. Thus, the functional TSLPR requires the IL-7Rα chain, but not the γc chain for signaling.

AB - The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Rα chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7Rα(-/-) mice, but did activate cells from γc(-/-) mice. Thus, the functional TSLPR requires the IL-7Rα chain, but not the γc chain for signaling.

KW - Cytokine

KW - Cytokine receptors

KW - DNA sequence

KW - Interleukin 2 receptor

KW - Interleukin 7 receptor

U2 - 10.1084/jem.192.5.659

DO - 10.1084/jem.192.5.659

M3 - Article

C2 - 10974032

SN - 1540-9538

SN - 0022-1007

VL - 192

SP - 659

EP - 669

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

IS - 5

ER -

Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor

Abstract

Keywords

UN SDGs

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