Cloning studies on the gene coding for l-(+)-lactate dehydrogenase of Plasmodium falciparum

David Ll Simmons; John E. Hyde; Martin Mackay; Mike Goman; John Scaife

doi:10.1016/0166-6851(85)90122-7

Cloning studies on the gene coding for l-(+)-lactate dehydrogenase of Plasmodium falciparum

David Ll Simmons, John E. Hyde, Martin Mackay, Mike Goman, John Scaife

Research output: Contribution to journal › Article › peer-review

Abstract

We show that the l-(+)-lactate dehydrogenase (EC 1.1.1.27; l-lactate; NAD+-oxidoreductase) of Plasmodium falciparum (LDH-P) is encoded in the parasite genome. A monoclonal antibody (McAb 7.2) has been shown to bind the LDH-P subunit which has an apparent molecular mass of 35 kDa. A polyclonal antiserum raised against affinity purified LDH-P has been used to isolate cDNA clones containing LDH-P epitopes from a λgt11Tn5 expression library. DNA sequence analysis of one clone, λLDH-P.1, reveals a single open reading frame which shows a degree of homology to the N-terminal domain of known LDH amino acid sequences. © 1985.

Original language	English
Pages (from-to)	231-243
Number of pages	12
Journal	Molecular and biochemical parasitology
Volume	15
Issue number	2
DOIs	https://doi.org/10.1016/0166-6851(85)90122-7
Publication status	Published - May 1985

Keywords

l-(+)-Lactate dehydrogenase
LDH clones
Malaria
Parasite enzymes
Plasmodium falciparum

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0166-6851(85)90122-7

Cite this

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title = "Cloning studies on the gene coding for l-(+)-lactate dehydrogenase of Plasmodium falciparum",

abstract = "We show that the l-(+)-lactate dehydrogenase (EC 1.1.1.27; l-lactate; NAD+-oxidoreductase) of Plasmodium falciparum (LDH-P) is encoded in the parasite genome. A monoclonal antibody (McAb 7.2) has been shown to bind the LDH-P subunit which has an apparent molecular mass of 35 kDa. A polyclonal antiserum raised against affinity purified LDH-P has been used to isolate cDNA clones containing LDH-P epitopes from a λgt11Tn5 expression library. DNA sequence analysis of one clone, λLDH-P.1, reveals a single open reading frame which shows a degree of homology to the N-terminal domain of known LDH amino acid sequences. {\textcopyright} 1985.",

keywords = "l-(+)-Lactate dehydrogenase, LDH clones, Malaria, Parasite enzymes, Plasmodium falciparum",

author = "Simmons, {David Ll} and Hyde, {John E.} and Martin Mackay and Mike Goman and John Scaife",

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T1 - Cloning studies on the gene coding for l-(+)-lactate dehydrogenase of Plasmodium falciparum

AU - Simmons, David Ll

AU - Hyde, John E.

AU - Mackay, Martin

AU - Goman, Mike

AU - Scaife, John

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Y1 - 1985/5

N2 - We show that the l-(+)-lactate dehydrogenase (EC 1.1.1.27; l-lactate; NAD+-oxidoreductase) of Plasmodium falciparum (LDH-P) is encoded in the parasite genome. A monoclonal antibody (McAb 7.2) has been shown to bind the LDH-P subunit which has an apparent molecular mass of 35 kDa. A polyclonal antiserum raised against affinity purified LDH-P has been used to isolate cDNA clones containing LDH-P epitopes from a λgt11Tn5 expression library. DNA sequence analysis of one clone, λLDH-P.1, reveals a single open reading frame which shows a degree of homology to the N-terminal domain of known LDH amino acid sequences. © 1985.

AB - We show that the l-(+)-lactate dehydrogenase (EC 1.1.1.27; l-lactate; NAD+-oxidoreductase) of Plasmodium falciparum (LDH-P) is encoded in the parasite genome. A monoclonal antibody (McAb 7.2) has been shown to bind the LDH-P subunit which has an apparent molecular mass of 35 kDa. A polyclonal antiserum raised against affinity purified LDH-P has been used to isolate cDNA clones containing LDH-P epitopes from a λgt11Tn5 expression library. DNA sequence analysis of one clone, λLDH-P.1, reveals a single open reading frame which shows a degree of homology to the N-terminal domain of known LDH amino acid sequences. © 1985.

KW - l-(+)-Lactate dehydrogenase

KW - LDH clones

KW - Malaria

KW - Parasite enzymes

KW - Plasmodium falciparum

U2 - 10.1016/0166-6851(85)90122-7

DO - 10.1016/0166-6851(85)90122-7

M3 - Article

C2 - 3892292

SN - 0166-6851

VL - 15

SP - 231

EP - 243

JO - Molecular and biochemical parasitology

JF - Molecular and biochemical parasitology

IS - 2

ER -

Cloning studies on the gene coding for l-(+)-lactate dehydrogenase of Plasmodium falciparum

Abstract

Keywords

UN SDGs

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