TY - JOUR
T1 - Comparative phenotype of circulating versus tissue immune cells in human lung and blood compartments during health and disease
AU - Colombo, Stefano A P
AU - Brown, Sheila L
AU - Hepworth, Matthew R
AU - Hankinson, Jenny
AU - Granato, Felice
AU - Kitchen, Semra J
AU - Hussell, Tracy
AU - Simpson, Angela
AU - Cook, Peter C
AU - MacDonald, Andrew S
N1 - © The Author(s) 2023. Published by Oxford University Press on behalf of the British Society for Immunology.
PY - 2023/7/19
Y1 - 2023/7/19
N2 - The lung is a dynamic mucosal surface constantly exposed to a variety of immunological challenges including harmless environmental antigens, pollutants, and potentially invasive microorganisms. Dysregulation of the immune system at this crucial site is associated with a range of chronic inflammatory conditions including asthma and Chronic Pulmonary Obstructive Disease (COPD). However, due to its relative inaccessibility, our fundamental understanding of the human lung immune compartment is limited. To address this, we performed flow cytometric immune phenotyping of human lung tissue and matched blood samples that were isolated from 115 donors undergoing lung tissue resection. We provide detailed characterization of the lung mononuclear phagocyte and T cell compartments, demonstrating clear phenotypic differences between lung tissue cells and those in peripheral circulation. Additionally, we show that CD103 expression demarcates pulmonary T cells that have undergone recent TCR and IL-7R signalling. Unexpectedly, we discovered that the immune landscape from asthmatic or COPD donors was broadly comparable to controls. Our data provide a much-needed expansion of our understanding of the pulmonary immune compartment in both health and disease.
AB - The lung is a dynamic mucosal surface constantly exposed to a variety of immunological challenges including harmless environmental antigens, pollutants, and potentially invasive microorganisms. Dysregulation of the immune system at this crucial site is associated with a range of chronic inflammatory conditions including asthma and Chronic Pulmonary Obstructive Disease (COPD). However, due to its relative inaccessibility, our fundamental understanding of the human lung immune compartment is limited. To address this, we performed flow cytometric immune phenotyping of human lung tissue and matched blood samples that were isolated from 115 donors undergoing lung tissue resection. We provide detailed characterization of the lung mononuclear phagocyte and T cell compartments, demonstrating clear phenotypic differences between lung tissue cells and those in peripheral circulation. Additionally, we show that CD103 expression demarcates pulmonary T cells that have undergone recent TCR and IL-7R signalling. Unexpectedly, we discovered that the immune landscape from asthmatic or COPD donors was broadly comparable to controls. Our data provide a much-needed expansion of our understanding of the pulmonary immune compartment in both health and disease.
UR - https://www.mendeley.com/catalogue/81edbbb0-499b-304c-b01c-b3e94c1cb25e/
U2 - 10.1093/discim/kyad009
DO - 10.1093/discim/kyad009
M3 - Article
C2 - 37545765
SN - 2754-2483
VL - 2
JO - Discovery Immunology
JF - Discovery Immunology
IS - 1
M1 - kyad009
ER -