Comparative studies of type X collagen expression in normal and rachitic chicken epiphyseal cartilage

A. P L Kwan, I. R. Dickson, A. J. Freemont, M. E. Grant

    Research output: Contribution to journalArticlepeer-review


    The levels of type X collagen in mineralizing normal chicken epiphyses and nonmineralizing rachitic chicken tibial epiphyses were measured and compared. Qualitative immunoperoxidase studies with anti-chick type X collagen monoclonal antibodies on sections from normal and rachitic cartilage demonstrated that the type X collagen levels in rachitic growth plates are reduced. Northern hybridization of mRNA and biosynthetic studies have confirmed that type X collagen synthesis in rickets is also decreased. In hypocalcemic rickets, the level of type X collagen mRNA is reduced by 80% whereas the level of type X collagen mRNA is only reduced by 50% in normocalcemic rickets. These observations provide additional evidence that type X collagen is involved in the process of cartilage mineralization and also suggest that the partial recovery of type X collagen synthesis in normocalcemic rickets may be related to the elevated plasma concentration of calcium. Calcium concentration may therefore play an important role in the control of type X collagen synthesis.
    Original languageEnglish
    Pages (from-to)1849-1856
    Number of pages7
    JournalJournal of Cell Biology
    Issue number4 I
    Publication statusPublished - 1989


    • Animals
    • diagnostic use: Antibodies, Monoclonal
    • Blotting, Northern
    • Chickens
    • analysis: Collagen
    • Gene Expression
    • cytology: Growth Plate
    • Immunoenzyme Techniques
    • Nucleic Acid Hybridization
    • genetics: RNA
    • Reference Values
    • Research Support, Non-U.S. Gov't
    • metabolism: Rickets


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