TY - JOUR
T1 - Comparative study of the production of rhamnolipid biosurfactants by B. thailandensis E264 and P. aeruginosa ATCC 9027 using foam fractionation
AU - Diaz De Rienzo, Mayri
AU - Kamalanathan, Ishara
AU - Martin, Peter
PY - 2016/7
Y1 - 2016/7
N2 - Biosurfactants are surface-active agents that are produced by a variety of microorganisms including yeasts, filamentous fungi and bacteria. In this work, we report on the ability of Pseudomonas aeruginosa ATCC 9027 and Burkholderia thailandensis E264 to produce rhamnolipids via a 10-L bioreactor and their recovery through foam fractionation studies in a continuous stripping mode. The recovery of Rha-C10-C10 (mono-rhamnolipids) produced by P. aeruginosa ATCC 9027 increased (from 6% to 96%), whilst the enrichment decreased (from 2.9 to 1.2) with the increasing airflow rate. These results are consistent with foam fractionation of a single surfactant system with stable foam. The recovery and enrichment of Rha-Rha-C14-C14 (di-rhamnolipids) produced by B. thailandensis E264 (and an unknown molecule) in a single-component system were found to display different characteristics. Both recovery and enrichment were found to decrease with the airflow rate. It is postulated that a competitive adsorption process could occur between the smaller molecule identified by electrospray ionisation–mass spectrometry (ESI–MS) and Rha-Rha-C14-C14.
AB - Biosurfactants are surface-active agents that are produced by a variety of microorganisms including yeasts, filamentous fungi and bacteria. In this work, we report on the ability of Pseudomonas aeruginosa ATCC 9027 and Burkholderia thailandensis E264 to produce rhamnolipids via a 10-L bioreactor and their recovery through foam fractionation studies in a continuous stripping mode. The recovery of Rha-C10-C10 (mono-rhamnolipids) produced by P. aeruginosa ATCC 9027 increased (from 6% to 96%), whilst the enrichment decreased (from 2.9 to 1.2) with the increasing airflow rate. These results are consistent with foam fractionation of a single surfactant system with stable foam. The recovery and enrichment of Rha-Rha-C14-C14 (di-rhamnolipids) produced by B. thailandensis E264 (and an unknown molecule) in a single-component system were found to display different characteristics. Both recovery and enrichment were found to decrease with the airflow rate. It is postulated that a competitive adsorption process could occur between the smaller molecule identified by electrospray ionisation–mass spectrometry (ESI–MS) and Rha-Rha-C14-C14.
U2 - 10.1016/j.procbio.2016.04.007
DO - 10.1016/j.procbio.2016.04.007
M3 - Article
SN - 1359-5113
VL - 51
SP - 820
EP - 827
JO - PROCESS BIOCHEMISTRY
JF - PROCESS BIOCHEMISTRY
IS - 7
ER -